Acute effects of UVB radiation on the proliferation and differentiation of keratinocytes

被引：64

作者：

Lee, JH ^{[1
]}

An, HT ^{[1
]}

Chung, JH ^{[1
]}

Kim, KH ^{[1
]}

Eun, HC ^{[1
]}

Cho, KH ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Med,Clin Res Inst, Dept Dermatol,Lab Cutaneous Ageing Res, Seoul Natl Univ Hosp, Seoul 110744, South Korea

来源：

PHOTODERMATOLOGY PHOTOIMMUNOLOGY & PHOTOMEDICINE | 2002年 / 18卷 / 05期

关键词：

UVB; keratinocytes; proliferation; differentiation;

D O I：

10.1034/j.1600-0781.2002.02755.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Purpose: The effects of UVB radiation on the proliferation and differentiation of epidermal keratinocytes were investigated with respect to timing, dosage, and repeated exposures. Methods: Nine healthy volunteers were placed into three subgroups and exposed to UVB radiation on buttock skin using a Waldmann UV 800 unit fitted with Philips TL-20W/12 fluorescent lamps. Three volunteers were given 2 MED of UVB and biopsied at: pre-exposure, 24, 48 and 72 h after UVB exposure. For three volunteers, 1 MED, 2 MED, 3 MED of UVB were applied. After 48 h, biopsies were taken from non-irradiated and irradiated sites. Finally, three volunteers received 1 MED of UVB daily for 5 days, and the non-irradiated and irradiated sites were biopsied 48 h after the final exposure. The expression of proliferation and differentiation markers by keratinocytes were detected by immunohistochemical staining, and the results were analysed quantitatively by image analysis. Results: The expression of proliferation and differentiation markers was observed prominently 48 h after irradiation. Higher doses of UVB caused an increase in proliferation and differentiation marker expression. Repeated exposures potentiated the effect of UVB radiation. Conclusion: UVB irradiation concomitantly promotes epidermal proliferation and differentiation. Responses were maximal 48 h after irradiation. This effect of UVB increases linearly according to dose and repetition.

引用

页码：253 / 261

页数：9

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