Benzyl Isothiocyanate and Phenethyl Isothiocyanate Inhibit Adipogenesis and Hepatosteatosis in Mice with Obesity Induced by a High-Fat Diet

被引：33

作者：

Chuang, Wei-Ting ^{[1
]}

Liu, Yun-Ta ^{[1
]}

Huang, Chin-Shiu ^{[2
]}

Lo, Chia-Wen ^{[1
]}

Yao, Hsien-Tsung ^{[1
]}

Chen, Haw-Wen ^{[1
]}

Lii, Chong-Kuei ^{[1
,2
]}

机构：

[1] China Med Univ, Dept Nutr, Taichung 404, Taiwan

[2] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung 413, Taiwan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2019年 / 67卷 / 25期

关键词：

benzyl isothiocyanate; phenethyl isothiocyanate; obesity; hepatosteatosis; pharmacokinetic; MITOTIC CLONAL EXPANSION; NF-KAPPA-B; ADIPOCYTE DIFFERENTIATION; GENE-EXPRESSION; C/EBP-BETA; INSULIN-RESISTANCE; LIPID-METABOLISM; ADIPOSE-TISSUE; PPAR-GAMMA; IN-VIVO;

D O I：

10.1021/acs.jafc.9b02668

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Benzyl isothiocyanate (BITC) and phenethyl isothiocyanate (PEITC) are organosulfur phytochemicals rich in cruciferous vegetables. We investigated the antiobesity and antihepatosteatosis activities of BITC and PEITC and the working mechanisms involved. C57BL/6J mice were fed a low-fat diet (LFD), a high-fat diet (HFD), or a HFD supplemented with 0.5 (L) or 1 g/kg (H) BITC or PEITC for 18 weeks. Compared with the HFD group, BITC or PEITC decreased the final body weight of mice in a dose-dependent manner [39.0 +/- 3.1 (HFD), 34.4 +/- 3.2 (BITC-L), 32.4 +/- 2.8 (BITC-H), 36.2 +/- 4.4 (PEITC-L), and 32.8 +/- 2.9 (PEITC-H) g, p < 0.05], relative weight of epididymal fat [5.7 +/- 0.4 (HFD), 4.7 +/- 0.7 (BITC-L), 3.7 +/- 0.3 (BITC-H), 4.4 +/- 1.0 (PEITC-L), and 3.2 +/- 0.6 (PEITC-H) %, p < 0.05], hepatic triglycerides [98.4 +/- 6.0 (HFD), 81.0 +/- 8.9 (BITC-L), 63.5 +/- 5.6 (BITC-H), 69.3 +/- 5.6 (PEITC-L), and 49.4 +/- 2.9 (PEITC-H) mg/g, p < 0.05], and plasma total cholesterol [140 +/- 21.3 (HFD), 109 +/- 5.6 (BITC-L), 101 +/- 11.3 (BITC-H), 126 +/- 8.3 (PEITC-L), and 91.8 +/- 12.7 (PEITC-H) mg/dL,p < 0.05]. Q-PCR and immunoblotting assays revealed that BITC and PEITC suppressed the expression of liver X receptor alpha, sterol regulatory element-binding protein 1c, stearoyl-CoA desaturase 1, fatty acid synthase, and acetyl-CoA carboxylase in both epididymal adipose and liver tissues. After a single oral administration of 85 mg/kg BITC or PEITC, the maximum plasma concentrations (C-max) of BITC and PEITC were 5.8 +/- 2.0 mu g/mL and 4.3 +/- 1.9 mu g/mL, respectively. In 3T3-L1 adipocytes, BITC and PEITC dose-dependently reduced adipocyte differentiation and cell cycle was arrested in G0/G1 phase. These findings indicate that BITC and PEITC ameliorate HFD-induced obesity and fatty liver by down-regulating adipocyte differentiation and the expression of lipogenic transcription factors and enzymes.

引用

页码：7136 / 7146

页数：11

共 49 条

[21] Benzyl isothiocyanate exhibits anti-inflammatory effects in murine macrophages and in mouse skin [J].