Multiple site-specific binding of Fis protein to Escherichia coli nuoA-N promoter DNA and its impact on DNA topology visualised by means of scanning force microscopy

被引：9

作者：

Zhang, J

Zeuner, Y

Kleefeld, A

Unden, G

Janshoff, A

机构：

[1] Johannes Gutenberg Univ Mainz, Inst Phys Chem, D-55128 Mainz, Germany

[2] Johannes Gutenberg Univ Mainz, Inst Mikrobiol & Weinforsch, D-55128 Mainz, Germany

来源：

CHEMBIOCHEM | 2004年 / 5卷 / 09期

关键词：

DNA; E; coli; Fis; nuo promoter; proteins; scanning probe microscopy;

D O I：

10.1002/cbic.200400022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins under wraps. The multiple site-specific binding of Fis (Factor for inversion stimulation) to DNA has been investigated by scanning force microscopy. Up to three Fis proteins bind to DNA in the region of the nuo promoter (see figure). Wrapping of the DNA around the protein complexes can be inferred from the compaction of the DNA.

引用

页码：1286 / 1289

页数：4

共 6 条

[1] THE N-TERMINAL PART OF THE ESCHERICHIA-COLI DNA-BINDING PROTEIN FIS IS ESSENTIAL FOR STIMULATING SITE-SPECIFIC DNA INVERSION BUT IS NOT REQUIRED FOR SPECIFIC DNA-BINDING
KOCH, C
NINNEMANN, O
FUSS, H
KAHMANN, R
NUCLEIC ACIDS RESEARCH, 1991, 19 (21) : 5915 - 5922
[2] ESCHERICHIA-COLI DNA TOPOISOMERASE-III IS A SITE-SPECIFIC DNA-BINDING PROTEIN THAT BINDS ASYMMETRICALLY TO ITS CLEAVAGE SITE
ZHANG, HL
MALPURE, S
DIGATE, RJ
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (40) : 23700 - 23705
[3] Site-specific binding of the 9.5 kilodalton DNA-binding protein ORF80 visualized by atomic force microscopy
Lysetska, M
Zettl, H
Oka, I
Lipps, G
Krauss, G
Krausch, G
BIOMACROMOLECULES, 2005, 6 (03) : 1252 - 1257
[4] ELONGATION BY ESCHERICHIA-COLI RNA-POLYMERASE IS BLOCKED INVITRO BY A SITE-SPECIFIC DNA-BINDING PROTEIN
PAVCO, PA
STEEGE, DA
JOURNAL OF BIOLOGICAL CHEMISTRY, 1990, 265 (17) : 9960 - 9969
[5] Insights into the architecture and stoichiometry of Escherichia coli PepADNA complexes involved in transcriptional control and site-specific DNA recombination by atomic force microscopy
Le Minh, Phu Nguyen
Devroede, Neel
Massant, Jan
Maes, Dominique
Charlier, Daniel
NUCLEIC ACIDS RESEARCH, 2009, 37 (05) : 1463 - 1476
[6] COOPERATIVE NON-SPECIFIC DNA-BINDING OF THE N-TERMINAL CORE OF THE CYCLIC-AMP RECEPTOR PROTEIN OF ESCHERICHIA-COLI AND ITS MODULATION BY CYCLIC-AMP
CLORE, GM
GRONENBORN, AM
DAVIES, RW
FEBS LETTERS, 1983, 164 (01) : 57 - 62

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