Potentiometric biosensing of proteins with ultrasensitive ion-selective microelectrodes and nanoparticle labels

被引：121
作者：
Chumbimuni-Torres, Karin Y.
Dai, Zong
Rubinova, Nastassia
Xiang, Yun
Pretsch, Erno [1 ]
Wang, Joseph
Bakker, Eric
机构：
[1] ETH, Organ Chem Lab, CH-8093 Zurich, Switzerland
[2] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
[3] Arizona State Univ, Biodesign Inst, Tempe, AZ 85287 USA
[4] Arizona State Univ, Fulton Sch Engn, Tempe, AZ 85287 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 42期
关键词：
D O I：
10.1021/ja065899k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report here for the first time on the use of potentiometry for ultrasensitive nanoparticle-based detection of protein interactions. A silver ion-selective microelectrode is used to detect silver ions oxidatively released from silver enlarged gold nanoparticle labels in a sandwich immunoassay. Since potentiometry is expected to largely maintain its analytical characteristics upon reducing the sample volume, it is anticipated that this approach may form the basis for bioassays with attractive detection limits. Copyright © 2006 American Chemical Society.
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页码：13676 / 13677
页数：2
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