Comprehensive and quantitative analysis of yeast deletion mutants defective in apical and isotropic bud growth

被引：40

作者：

Watanabe, Machika ^{[1
]}

Watanabe, Daisuke ^{[1
]}

Nogami, Satoru ^{[1
]}

Morishita, Shinichi ^{[2
]}

Ohya, Yoshikazu ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Chiba 2778562, Japan

[2] Univ Tokyo, Grad Sch Frontier Sci, Dept Computat Biol, Chiba 2778562, Japan

来源：

CURRENT GENETICS | 2009年 / 55卷 / 04期

关键词：

Cell morphology; Apical growth; Polarized growth; Actin; Yeast; CELL-CYCLE CONTROL; SACCHAROMYCES-CEREVISIAE; BUDDING YEAST; POLARIZED GROWTH; GENE ONTOLOGY; MORPHOGENESIS; PROTEIN; COMPLEX; ACTIN; DISSECTION;

D O I：

10.1007/s00294-009-0251-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To obtain a comprehensive understanding of the budding phase transition, 4,711 Saccharomyces cerevisiae haploid nonessential gene deletion mutants were screened with the image processing program CalMorph, and 35 mutants with a round bud and 173 mutants with an elongated bud were statistically identified. We classified round and elongated bud mutants based on factors thought to affect the duration of the apical bud growth phase. Two round bud mutants (arc18 and sac6) were found to be defective in apical actin patch localization. Several elongated bud mutants demonstrated a delay of cell cycle progression at the apical growth phase, suggesting that these mutants have a defect in the control of cell cycle progression.

引用

页码：365 / 380

页数：16

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