Hormone activation induces nucleosome positioning in vivo

被引:55
作者
Belikov, S
Gelius, B
Almouzni, G
Wrange, Ö
机构
[1] Karolinska Inst, Med Nobel Inst, Dept Cell & Mol Biol, Mol Genet Lab, SE-17177 Stockholm, Sweden
[2] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 117984, Russia
[3] Inst Curie, UMR 218 CNRS, FR-75231 Paris 05, France
关键词
chromatin structure; glucocorticoid receptor; MMTV promoter; nucleosome positioning; Xenopus oocyte;
D O I
10.1093/emboj/19.5.1023
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The mouse mammary tumor virus (MMTV) promoter is induced by glucocorticoid hormone. A robust hormone- and receptor-dependent activation could be reproduced in Xenopus laevis oocytes. The homogeneous response in this system allowed a detailed analysis of the transition in chromatin structure following hormone activation. This revealed two novel findings: hormone activation led to the establishment of specific translational positioning of nucleosomes despite the lack of significant positioning in the inactive state; and, in the active promoter, a subnucleosomal particle encompassing the glucocorticoid receptor (GR)-binding region was detected. The presence of only a single GR-binding site was sufficient for the structural transition to occur, Both basal promoter elements and ongoing transcription were dispensable. These data reveal a stepwise process in the transcriptional activation by glucocorticoid hormone.
引用
收藏
页码:1023 / 1033
页数:11
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