Epigallocatechin-3-O-gallate up-regulates microRNA-let-7b expression by activating 67-kDa laminin receptor signaling in melanoma cells

被引：79

作者：

Yamada, Shuhei ^{[1
]}

Tsukamoto, Shuntaro ^{[1
]}

Huang, Yuhui ^{[1
]}

Makio, Akiko ^{[1
]}

Kumazoe, Motofumi ^{[1
]}

Yamashita, Shuya ^{[1
]}

Tachibana, Hirofumi ^{[1
]}

机构：

[1] Kyushu Univ, Fac Agr, Dept Biosci & Biotechnol, Div Appl Biol Chem, Fukuoka 8128581, Japan

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

日本学术振兴会;

关键词：

PROTEIN PHOSPHATASE 2A; HUMAN PROSTATE-CANCER; TEA POLYPHENOL EGCG; GREEN-TEA; CHEMOPREVENTION; INHIBITION; CATECHINS; LET-7; HMGA2;

D O I：

10.1038/srep19225

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

MicroRNAs (miRNAs) are non-coding RNAs involved in various biological processes by regulating their target genes. Green tea polyphenol (-)-epigallocatechin-3-O-gallate (EGCG) inhibits melanoma tumor growth by activating 67-kDa laminin receptor (67LR) signaling. To examine the effect of EGCG on miRNA expression in melanoma cells, we performed miRNA microarray analysis. We showed that EGCG up-regulated miRNA-let-7b expression through 67LR in melanoma cells. The EGCG-induced upregulation of let-7b led to down-regulation of high mobility group A2 (HMGA2), a target gene related to tumor progression. 67LR-dependent cAMP/protein kinase A (PKA)/protein phosphatase 2A (PP2A) signaling pathway activation was involved in the up-regulation of let-7b expression induced by EGCG. These findings provide a basis for understanding the mechanism of miRNA regulation by EGCG.

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页数：8

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