Polydispersity in size-exclusion chromatography: A stochastic approach

被引:8
作者
Sepsey, Annamaria [1 ]
Bacskay, Ivett [2 ,3 ]
Felinger, Attila [1 ,2 ,3 ]
机构
[1] MTA PTE Mol Interact Separat Sci Res Grp, H-7624 Pecs, Hungary
[2] Univ Pecs, Dept Analyt & Environm Chem, H-7624 Pecs, Hungary
[3] Univ Pecs, Szentagothai Res Ctr, H-7624 Pecs, Hungary
关键词
Polydispersity; Stochastic theory; Size-exclusion chromatography; GEL-PERMEATION CHROMATOGRAPHY; CALIBRATION; SEPARATION; RETENTION; SINGLE;
D O I
10.1016/j.chroma.2014.09.023
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We investigate the impact of polydispersity of the sample molecules on the separation process and on the efficiency of size-exclusion chromatography. Polydispersity was integrated into the molecular (stochastic) model of chromatography; the characteristic function, the band profile and the most important moments of the elution profiles were calculated for several kind of pore structures. We investigated the parameters affected by polydispersity on the separation for a number of pore shapes. Our results demonstrate that even a small distribution in the molecular size (i.e. polydispersity) can contribute substantially to the total width of the chromatographic peak. The pure effect of polydispersity can only be investigated via mathematical modeling, because its contribution to an experimental chromatogram cannot be separated from other band-broadening effects. (C)2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:156 / 163
页数:8
相关论文
共 25 条
[1]   Determination of the pore size distribution of high-performance liquid chromatography stationary phases via inverse size exclusion chromatography [J].
Bacskay, Ivett ;
Sepsey, Annamaria ;
Felinger, Attila .
JOURNAL OF CHROMATOGRAPHY A, 2014, 1339 :110-117
[2]   DETERMINATION OF THEORETICAL PLATES IN GEL PERMEATION CHROMATOGRAPHY BY USING POLYDISPERSE MATERIALS (POLYMERS) [J].
BLY, DD .
JOURNAL OF POLYMER SCIENCE PART A-1-POLYMER CHEMISTRY, 1968, 6 (8PA1) :2085-&
[3]   UNIQUE PROPERTIES AND USES OF A LINEAR SEMI-LOG CALIBRATION IN GEL PERMEATION CHROMATOGRAPHY [J].
BLY, DD .
ANALYTICAL CHEMISTRY, 1969, 41 (03) :477-&
[4]  
Carmichael J. B., 1968, MACROMOLECULES, V1, P526
[5]   STOCHASTIC MODEL FOR GEL PERMEATION SEPARATION OF POLYMERS [J].
CARMICHAEL, JB .
JOURNAL OF POLYMER SCIENCE PART A-2-POLYMER PHYSICS, 1968, 6 (3PA2) :517-+
[6]   THEORY OF GEL FILTRATION SEPARATION OF BIOPOLYMERS ASSUMING A GAUSSIAN DISTRIBUTION OF PORE SIZES [J].
CARMICHAEL, JB .
BIOPOLYMERS, 1968, 6 (10) :1497-+
[7]   Stochastic theory of multiple-site linear adsorption chromatography [J].
Cavazzini, A ;
Remelli, M ;
Dondi, F ;
Felinger, A .
ANALYTICAL CHEMISTRY, 1999, 71 (16) :3453-3462
[8]   THE CHARACTERISTIC FUNCTION-METHOD IN THE STOCHASTIC-THEORY OF CHROMATOGRAPHY [J].
DONDI, F ;
REMELLI, M .
JOURNAL OF PHYSICAL CHEMISTRY, 1986, 90 (09) :1885-1891
[9]   Stochastic theory of size exclusion chromatography by the characteristic function approach [J].
Dondi, F ;
Cavazzini, A ;
Remelli, M ;
Felinger, A ;
Martin, M .
JOURNAL OF CHROMATOGRAPHY A, 2002, 943 (02) :185-207
[10]   Stochastic theory of size exclusion chromatography: Peak shape analysis on single columns [J].
Felinger, A ;
Pasti, L ;
Dondi, F ;
van Hulst, M ;
Schoenmakers, PJ ;
Martin, M .
ANALYTICAL CHEMISTRY, 2005, 77 (10) :3138-3148