Regulation of Corynebacterium glutamicum Heat Shock Response by the Extracytoplasmic-Function Sigma Factor SigH and Transcriptional Regulators HspR and HrcA

被引:58
作者
Ehira, Shigeki [1 ]
Teramoto, Haruhiko [1 ]
Inui, Masayuki [1 ]
Yukawa, Hideaki [1 ]
机构
[1] Res Inst Innovat Technol Earth, RITE, Kyoto 6190292, Japan
关键词
STREPTOMYCES-ALBUS G; OXYGEN-DEPRIVATION CONDITIONS; GRAM-POSITIVE BACTERIA; MYCOBACTERIUM-TUBERCULOSIS; BACILLUS-SUBTILIS; GENE-EXPRESSION; DNAK OPERON; NEGATIVE REGULATOR; COELICOLOR A3(2); GENOME SEQUENCE;
D O I
10.1128/JB.00112-09
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Heat shock response in Corynebacterium glutamicum was characterized by whole-genome expression analysis using a DNA microarray. It was indicated that heat shock response of C. glutamicum included not only upregulation of heat shock protein (HSP) genes encoding molecular chaperones and ATP-dependent proteases, but it also increased and decreased expression of more than 300 genes related to disparate physiological functions. An extracytoplasmic-function sigma factor, SigH, was upregulated by heat shock. The SigH regulon was defined by gene expression profiling using sigH-disrupted and overexpressing strains in conjunction with mapping of transcription initiation sites. A total of 45 genes, including HSP genes and genes involved in oxidative stress response, were identified as the SigH regulon. Expression of some HSP genes was also upregulated by deletion of the transcriptional regulators HspR and HrcA. HspR represses expression of the clpB and dnaK operons, and HrcA represses expression of groESL1 and groEL2. SigH was shown to play an important role in regulation of heat shock response in concert with HspR and HrcA, but its role is likely restricted to only a part of the regulation of C. glutamicum heat shock response. Upregulation of 18 genes encoding transcriptional regulators by heat shock suggests a complex regulatory network of heat shock response in C. glutamicum.
引用
收藏
页码:2964 / 2972
页数:9
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  • [1] RETRACTED: Heat shock Proteome analysis of wild-type Corynebacterium glutamicum ATCC 13032 and a spontaneous mutant lacking GroEL1, a dispensable chaperone (Retracted article. See vol. 195, pg. 2707, 2013)
    Barreiro, C
    González-Lavado, E
    Brand, S
    Tauch, A
    Martín, JF
    [J]. JOURNAL OF BACTERIOLOGY, 2005, 187 (03) : 884 - 889
  • [2] RETRACTED: Transcriptional analysis of the groES-groEL1, groEL2, and dnaK genes in Corynebacterium glutamicum:: Characterization of heat shock-induced promoters (Retracted article. See vol. 195, pg. 2706, 2013)
    Barreiro, C
    González-Lavado, E
    Pátek, M
    Martín, JF
    [J]. JOURNAL OF BACTERIOLOGY, 2004, 186 (14) : 4813 - 4817
  • [3] Regulation of AmtR-controlled gene expression in Corynebacterium glutamicum:: mechanism and characterization of the AmtR regulon
    Beckers, G
    Strösser, J
    Hildebrandt, U
    Kalinowski, J
    Farwick, M
    Krämer, R
    Burkovski, A
    [J]. MOLECULAR MICROBIOLOGY, 2005, 58 (02) : 580 - 595
  • [4] The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium efficiens, Corynebacterium diphtheriae and Corynebacterium jeikeium deduced from the complete genome sequences -: art. no. 86
    Brune, I
    Brinkrolf, K
    Kalinowski, J
    Pühler, A
    Tauch, A
    [J]. BMC GENOMICS, 2005, 6 (1)
  • [5] Negative feedback regulation of dnaK, clpB and lon expression by the DnaK chaperone machine in Streptomyces coelicolor, identified by transcriptome and in vivo DnaK-depletion analysis
    Bucca, G
    Brassington, AME
    Hotchkiss, G
    Mersinias, V
    Smith, CP
    [J]. MOLECULAR MICROBIOLOGY, 2003, 50 (01) : 153 - 166
  • [6] Global analysis of heat shock response in Desulfovibrio vulgaris Hildenborough
    Chhabra, SR
    He, Q
    Huang, KH
    Gaucher, SP
    Alm, EJ
    He, Z
    Hadi, MZ
    Hazen, TC
    Wall, JD
    Zhou, J
    Arkin, AP
    Singh, AK
    [J]. JOURNAL OF BACTERIOLOGY, 2006, 188 (05) : 1817 - 1828
  • [7] Modulation of DNA-binding activity of Mycobacterium tuberculosis HspR by chaperones
    Das Gupta, Twishasri
    Bandyopadhyay, Boudhayan
    Das Gupta, Sujoy K.
    [J]. MICROBIOLOGY-SGM, 2008, 154 : 484 - 490
  • [8] Redox control in actinobacteria
    den Hengst, Chris D.
    Buttner, Mark J.
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2008, 1780 (11): : 1201 - 1216
  • [9] Protein folding and degradation in bacteria: To degrade or not to degrade? That is the question
    Dougan, DA
    Mogk, A
    Bukau, B
    [J]. CELLULAR AND MOLECULAR LIFE SCIENCES, 2002, 59 (10) : 1607 - 1616
  • [10] TRANSCRIPTIONAL ANALYSIS OF GROEL GENES IN STREPTOMYCES-COELICOLOR A3(2)
    DUCHENE, AM
    THOMPSON, CJ
    MAZODIER, P
    [J]. MOLECULAR & GENERAL GENETICS, 1994, 245 (01): : 61 - 68