Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser

被引：0

作者：

Arnlund, David ^{[1
]}

Johansson, Linda C. ^{[1
]}

Wickstrand, Cecilia ^{[1
]}

Barty, Anton ^{[2
]}

Williams, Garth J. ^{[3
]}

Malmerberg, Erik ^{[1
]}

Davidsson, Jan ^{[4
]}

Milathianaki, Despina ^{[3
]}

DePonte, Daniel P. ^{[2
,3
]}

Shoeman, Robert L. ^{[5
,6
]}

Wang, Dingjie ^{[7
]}

James, Daniel ^{[7
]}

Katona, Gergely ^{[1
]}

Westenhoff, Sebastian ^{[1
]}

White, Thomas A. ^{[2
]}

Aquila, Andrew ^{[2
]}

Bari, Sadia ^{[6
,8
]}

Berntsen, Peter ^{[1
]}

Bogan, Mike ^{[9
]}

van Driel, Tim Brandt ^{[10
]}

Doak, R. Bruce ^{[5
,7
]}

Kjaer, Kasper Skov ^{[10
,11
]}

Frank, Matthias ^{[12
]}

Fromme, Raimund ^{[13
]}

Grotjohann, Ingo ^{[13
]}

Henning, Robert ^{[14
]}

Hunter, Mark S. ^{[13
]}

Kirian, Richard A. ^{[7
]}

Kosheleva, Irina ^{[14
]}

Kupitz, Christopher ^{[13
]}

Liang, Mengning ^{[2
]}

Martin, Andrew V. ^{[2
]}

Nielsen, Martin Meedom ^{[10
]}

Messerschmidt, Marc ^{[2
,3
]}

Seibert, M. Marvin ^{[3
]}

Sjohamn, Jennie ^{[1
]}

Stellato, Francesco ^{[2
]}

Weierstall, Uwe ^{[7
]}

Zatsepin, Nadia A. ^{[7
]}

Spence, John C. H. ^{[7
]}

Fromme, Petra ^{[13
]}

Schlichting, Ilme ^{[5
,6
]}

Boutet, Sebastien ^{[3
]}

Groenhof, Gerrit ^{[15
,16
]}

Chapman, Henry N. ^{[2
,17
,18
]}

Neutze, Richard ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden

[2] DESY, Ctr Free Electron Laser Sci, Hamburg, Germany

[3] SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA USA

[4] Uppsala Univ, Dept Chem, Angstrom Lab, Uppsala, Sweden

[5] Max Planck Inst Med Res, D-69120 Heidelberg 1, Germany

[6] Ctr Free Elect Laser Sci, Max Planck Adv Study Grp, Hamburg, Germany

[7] Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA

[8] Max Planck Inst Kernphys, D-69117 Heidelberg, Germany

[9] SLAC Natl Accelerator Lab, PULSE Inst Ultrafast Energy Sci, Menlo Pk, CA USA

[10] Tech Univ Denmark, Dept Phys, DK-2800 Lyngby, Denmark

[11] Univ Copenhagen, Niels Bohr Inst, DK-2100 Copenhagen, Denmark

[12] Lawrence Livermore Natl Lab, Livermore, CA USA

[13] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ USA

[14] Univ Chicago, BioCARS, Chicago, IL 60637 USA

[15] Univ Jyvaskyla, Nanosci Ctr, Jyvaskyla, Finland

[16] Univ Jyvaskyla, Dept Chem, Jyvaskyla, Finland

[17] Univ Hamburg, Dept Phys, Hamburg, Germany

[18] Ctr Ultrafast Imaging, Hamburg, Germany

来源：

NATURE METHODS | 2014年 / 11卷 / 09期

基金：

美国国家科学基金会; 芬兰科学院;

关键词：

FREQUENCY VIBRATIONAL-MODES; STRUCTURAL DYNAMICS;

D O I：

10.1038/NMETH.3067

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.

引用

页码：923 / 926

页数：4

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