Correcting field-dependent aberrations with nanoscale accuracy in three-dimensional single-molecule localization microscopy

被引：80
作者：
von Diezmann, Alex [1 ]
Lee, Maurice Y. [1 ,2 ]
Lew, Matthew D. [1 ,3 ]
Moerner, W. E. [1 ]
机构：
[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA
[2] Stanford Univ, Biophys Program, Stanford, CA 94305 USA
[3] Stanford Univ, Dept Elect Engn, Stanford, CA 94305 USA
来源：
OPTICA | 2015年 / 2卷 / 11期
基金：
美国国家科学基金会;
关键词：
OPTICAL RECONSTRUCTION MICROSCOPY; POINT-SPREAD FUNCTION; SUPERRESOLUTION MICROSCOPY; FLUORESCENCE MICROSCOPY; DIFFRACTION-LIMIT; FLUOROPHORE LOCALIZATION; DISTANCE MEASUREMENTS; PARTICLE TRACKING; LIVING CELLS; DIMENSIONS;
D O I：
10.1364/OPTICA.2.000985
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
The localization of single fluorescent molecules enables the imaging of molecular structure and dynamics with sub-diffraction precision and can be extended to three dimensions using point spread function (PSF) engineering. However, the nanoscale accuracy of localization throughout a 3D single-molecule microscope's field of view has not yet been rigorously examined. By using regularly spaced subdiffraction apertures filled with fluorescent dyes, we reveal field-dependent aberrations as large as 50-100 nm and show that they can be corrected to less than 25 nm over an extended 3D focal volume. We demonstrate the applicability of this technique for two engineered PSFs, the double-helix PSF and the astigmatic PSF. We expect these results to be broadly applicable to 3D single-molecule tracking and superresolution methods demanding high accuracy. (C) 2015 Optical Society of America
引用
收藏
页码：985 / 993
页数：9
相关论文
共 54 条
[1]  
Aquino D, 2011, NAT METHODS, V8, P353, DOI [10.1038/NMETH.1583, 10.1038/nmeth.1583]
[2]   Extending Single-Molecule Microscopy Using Optical Fourier Processing [J].
Backer, Adam S. ;
Moerner, W. E. .
JOURNAL OF PHYSICAL CHEMISTRY B, 2014, 118 (28) :8313-8329
[3]   The Role of Molecular Dipole Orientation in Single-Molecule Fluorescence Microscopy and Implications for Super-Resolution Imaging [J].
Backlund, Mikael P. ;
Lew, Matthew D. ;
Backer, Adam S. ;
Sahl, Steffen J. ;
Moerner, W. E. .
CHEMPHYSCHEM, 2014, 15 (04) :587-599
[4]   Simultaneous, accurate measurement of the 3D position and orientation of single molecules [J].
Backlund, Mikael P. ;
Lew, Matthew D. ;
Backer, Adam S. ;
Sahl, Steffen J. ;
Grover, Ginni ;
Agrawal, Anurag ;
Piestun, Rafael ;
Moerner, W. E. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (47) :19087-19092
[5]   4D Super-Resolution Microscopy with Conventional Fluorophores and Single Wavelength Excitation in Optically Thick Cells and Tissues [J].
Baddeley, David ;
Crossman, David ;
Rossberger, Sabrina ;
Cheyne, Juliette E. ;
Montgomery, Johanna M. ;
Jayasinghe, Isuru D. ;
Cremer, Christoph ;
Cannell, Mark B. ;
Soeller, Christian .
PLOS ONE, 2011, 6 (05)
[6]   DIFFUSION OF LOW-DENSITY LIPOPROTEIN-RECEPTOR COMPLEX ON HUMAN-FIBROBLASTS [J].
BARAK, LS ;
WEBB, WW .
JOURNAL OF CELL BIOLOGY, 1982, 95 (03) :846-852
[7]   Imaging intracellular fluorescent proteins at nanometer resolution [J].
Betzig, Eric ;
Patterson, George H. ;
Sougrat, Rachid ;
Lindwasser, O. Wolf ;
Olenych, Scott ;
Bonifacino, Juan S. ;
Davidson, Michael W. ;
Lippincott-Schwartz, Jennifer ;
Hess, Harald F. .
SCIENCE, 2006, 313 (5793) :1642-1645
[8]  
Born M., 1999, PRINCIPLES OPTICS
[9]   Simultaneous measurement of position and color of single fluorescent emitters using diffractive optics [J].
Broeken, Jordi ;
Rieger, Bernd ;
Stallinga, Sjoerd .
OPTICS LETTERS, 2014, 39 (11) :3352-3355
[10]   Adaptive optics correction of specimen-induced aberrations in single-molecule switching microscopy [J].
Burke, Daniel ;
Patton, Brian ;
Huang, Fang ;
Bewersdorf, Joerg ;
Booth, Martin J. .
OPTICA, 2015, 2 (02) :177-185
123456