The biomimetic deposition of hydroxyapatite (HA) on the surface of SiO2 thin film coated metal substrates was developed and investigated. The structural investigations of HA were made by XRD and FTIR-ATR, while morphological and chemical changes during HA biomimetic deposition on the surfaces of silica thin films were investigated by SEM with EDS. The HA film thicknesses were estimated from the mass changes of samples including the corresponding correction of the pore volume inside of them, which was calculated by the Lecloux and Pirard method based on the Dollimore-Heal method. (c) 2013 Elsevier Ltd and Techna Group S.r.l. All rights reserved.