Optimisation of a microsatellite enrichment technique in Saccharum spp.

被引：43

作者：

Cordeiro, GM

Maguire, TL

Edwards, KJ

Henry, RJ

机构：

[1] So Cross Univ, Ctr Plant Conservat Genet, Lismore, NSW 2480, Australia

[2] So Cross Univ, Ctr Coastal Management, Lismore, NSW 2480, Australia

[3] Univ Bristol, Dept Agr Sci, IACR, Long Ashton Res Stn, Bristol BS18 9AF, Avon, England

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1999年 / 17卷 / 03期

关键词：

enriched microsatellite library; molecular marker; Saccharum; SSR; sugarcane;

D O I：

10.1023/A:1007640231900

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The creation of enriched microsatellite libraries can ensure an abundant supply of microsatellite sequences at a considerably reduced cost. We report here the modification to an existing enrichment protocol and the optimisation of this technique for use on specific plant species, in this case, sugarcane. Using this optimised protocol, we achieved a 40% increase in the level of enrichment.

引用

页码：225 / 229

页数：5