Decreased Level of Albumin in Peripheral Blood Mononuclear Cells of Streptozotocin-Induced Diabetic Rats

被引：14

作者：

Park, Ki Tae ^{[1
]}

Yun, Chul-Ho ^{[1
]}

Bae, Chun-Sik ^{[2
]}

Ahn, Taeho ^{[2
]}

机构：

[1] Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea

[2] Chonnam Natl Univ, Coll Vet Med, Kwangju 500757, South Korea

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2014年 / 76卷 / 08期

关键词：

albumin; diabetes; expression; oxidative stress; streptozotocin; OXIDATIVE STRESS; RADICAL PRODUCTION; URINARY ALBUMIN; EXPRESSION; SERUM; MELLITUS; TISSUES; PROTEIN;

D O I：

10.1292/jvms.13-0631

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We investigated the phenotypic level of albumin in peripheral blood mononuclear cells (PBMC) of streptozotocin (STZ)-induced diabetic rats. A specific reduction of albumin was identified by 2-dimensional electrophoresis and mass spectrometry. Decreased albumin content was also confirmed by immunoblotting and quantitative real-time PCR. Since albumin is a major and predominant antioxidant in plasma, the PBMC albumin may also contribute to their antioxidant activity. By measuring the amount of H2O2, lipid peroxidation and the redox form of glutathione, it was found that the production of the oxidative stress was elevated in STZ-diabetic rats compared to that of normal control. We suggest, therefore, that decreased albumin content may lead to the decreased antioxidant activity in the PBMC of type 1 diabetic rats.

引用

页码：1087 / 1092

页数：6

共 25 条

[1] Human Microglial Cells Synthesize Albumin in Brain [J].

Ahn, Sung-Min ;

Byun, Kyunghee ;

Cho, Kun ;

Kim, Jin Young ;

Yoo, Jong Shin ;

Kim, Deokhoon ;

Paek, Sun Ha ;

Kim, Seung U. ;

Simpson, Richard J. ;

Lee, Bonghee .

PLOS ONE, 2008, 3 (07)

[2] Tissue-specitic effect of ascorbic acid supplementation on the expression of cytochrome P450 2E1 and oxidative stress in streptozotocin-induced diabetic rats [J].

Ahn, Taeho ;

Yun, Chul-Ho ;

Oh, Doo-Byoung .

TOXICOLOGY LETTERS, 2006, 166 (01) :27-36

[3] Role of oxidative stress in diabetic complications - A new perspective on an old paradigm [J].

Baynes, JW ;

Thorpe, SR .

DIABETES, 1999, 48 (01) :1-9

[4] The importance of proteins in defense against oxidation [J].

Bourdon, E ;

Blache, D .

ANTIOXIDANTS & REDOX SIGNALING, 2001, 3 (02) :293-311

[5] GLUTATHIONE ALTERATIONS IN RAT-LIVER AFTER ACUTE AND SUBACUTE ORAL-ADMINISTRATION OF PARACETAMOL [J].

BUTTAR, HS ;

CHOW, AYK ;

DOWNIE, RH .

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 1977, 4 (01) :1-6

[6] Xanthine oxidase is involved in free radical production in type 1 diabetes -: Protection by allopurinol [J].

Desco, MC ;

Asensi, A ;

Márquez, R ;

Martínez-Valls, J ;

Vento, M ;

Pallardó, FV ;

Sastre, J ;

Viña, J .

DIABETES, 2002, 51 (04) :1118-1124

[7]

DZIARSKI R, 1994, J BIOL CHEM, V269, P20431

[8] ELECTRICAL CHARGE OF SERUM AND URINARY ALBUMIN IN NORMAL AND DIABETIC HUMANS [J].

GHIGGERI, GM ;

CANDIANO, G ;

DELFINO, G ;

QUEIROLO, C .

KIDNEY INTERNATIONAL, 1985, 28 (02) :168-177

[9] HYDROXYL RADICAL PRODUCTION AND AUTOXIDATIVE GLYCOSYLATION - GLUCOSE AUTOXIDATION AS THE CAUSE OF PROTEIN DAMAGE IN THE EXPERIMENTAL GLYCATION MODEL OF DIABETES-MELLITUS AND AGING [J].

HUNT, JV ;

DEAN, RT ;

WOLFF, SP .

BIOCHEMICAL JOURNAL, 1988, 256 (01) :205-212

[10] The Ratio of the Blood Urea Nitrogen/Creatinine Index in Patients with Acute Renal Failure is Decreased due to Dextran or Mannitol [J].

Kouki, Tsuyoshi ;

Komiya, Ichiro ;

Masuzaki, Hiroaki .

INTERNAL MEDICINE, 2010, 49 (03) :223-226

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