Cathodic electrochemiluminescence immunosensor based on nanocomposites of semiconductor carboxylated g-C3N4 and graphene for the ultrasensitive detection of squamous cell carcinoma antigen

被引：164
作者：
Li, Xiaojian [1 ]
Zhang, Xiaoyue [1 ]
Ma, Hongmin [1 ]
Wu, Dan [1 ]
Zhang, Yong [1 ]
Du, Bin [1 ]
Wei, Qin [1 ]
机构：
[1] Univ Jinan, Key Lab Chem Sensing & Anal Univ Shandong, Sch Chem & Chem Engn, Jinan 250022, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2014年 / 55卷
关键词：
Electrochemiluminescence immunosensor; Carboxylated graphitic carbon nitride; Squamous cell carcinoma antigen; Graphene; CARBON NITRIDE; ELECTROGENERATED CHEMILUMINESCENCE; ELECTROCHEMICAL IMMUNOSENSOR; NANOPARTICLES; IMMUNOASSAY; IMMOBILIZATION; COMPOSITES; COREACTANT; REDUCTION; SURFACE;
D O I：
10.1016/j.bios.2013.12.039
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A novel label-free electrochemiluminescence (ECL) immunosensor was developed for the detection of squamous cell carcinoma antigen (SCCA) based on nanocomposites of semiconductor carboxylated graphitic carbon nitride (g-C3N4) and graphene (g-C3N4-graphene). The ECL intensity of carboxylated g-C3N4 was much enhanced after being combined with graphene which had excellent electron-transfer ability. The sensing platform was constructed by depositing g-C3N4-graphene on electrodes and immobilizing antibodies on the surface of carboxylated g-C3N4 through amidation. The specific immunoreaction between SCCA and antibody resulted in the decrease of ECL intensity and the intensity decreased linearly with the logarithm of SCCA concentration in the range of 0.025-10 ng mL(-1) with a detection limit of 8.53 pg mL(-1). The developed ECL immunosensor exhibited high sensitivity, good reproducibility and long-term stability, which possessed great potential for cancer detection in clinical laboratory diagnosis. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：330 / 336
页数：7
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