Enhanced 3D localization of individual RNA transcripts via astigmatic imaging

被引：0

作者：

Perillo, Evan P. ^{[1
]}

De Haro, Leyma ^{[2
]}

Phipps, Mary E. ^{[2
]}

Martinez, Jennifer S. ^{[2
]}

Yeh, Hsin-Chih ^{[1
]}

Dunn, Andrew K. ^{[1
]}

Shepherd, Douglas P. ^{[2
,3
]}

Werner, James H. ^{[2
]}

机构：

[1] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA

[2] Los Alamos Natl Lab, Ctr Intergrated Nanotechnol, Los Alamos, NM 87544 USA

[3] Univ Colorado, Dept Phys, Denver, CO 80204 USA

来源：

SINGLE MOLECULE SPECTROSCOPY AND SUPERRESOLUTION IMAGING VII | 2014年 / 8950卷

关键词：

smFISH; single-molecule localization; astigmatic imaging; fluorescence microscopy; gene expression; GENE-EXPRESSION; STEM-CELLS; SINGLE; OSTEOBLAST; REVEALS; DIFFERENTIATION;

D O I：

10.1117/12.2038197

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Here we present an automated microscope capable of 3D multi-color single molecule localization of individual messenger RNA molecules in a wide range of cell types. We have implemented astigmatic imaging with a cylindrical lens to improve z-localization, and a maximum likelihood estimator on a graphics processing unit to improve localization precision and speed. This microscope will aid in gene expression analysis by its capability to perform high throughput imaging of thick cells and tissues while still maintaining sufficient z resolution to resolve single RNA transcripts in three dimensions. Enhanced z-localization allows for resolving membrane localized and co-localized transcripts.

引用

页数：11

共 50 条

[11] Observation of live chromatin dynamics in cells via 3D localization microscopy using Tetrapod point spread functions
Shechtman, Yoav
Gustavssonn, ANNA-Karin
Petrov, Petar N.
Dultz, Elisa
Lee, Maurice Y.
Weis, Karsten
Moerner, W. E.
BIOMEDICAL OPTICS EXPRESS, 2017, 8 (12): : 5735 - 5748
[12] 3D structures of individual mammalian genomes studied by single-cell Hi-C
Stevens, Tim J.
Lando, David
Basu, Srinjan
Atkinson, Liam P.
Cao, Yang
Lee, Steven F.
Leeb, Martin
Wohlfahrt, Kai J.
Boucher, Wayne
O'Shaughnessy-Kirwan, Aoife
Cramard, Julie
Faure, Andre J.
Ralser, Meryem
Blanco, Enrique
Morey, Lluis
Sanso, Miriam
Palayret, Matthieu G. S.
Lehner, Ben
Di Croce, Luciano
Wutz, Anton
Hendrich, Brian
Klenerman, Dave
Laue, Ernest D.
NATURE, 2017, 544 (7648) : 59 - +
[13] ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range
Aristov, Andrey
Lelandais, Benoit
Rensen, Elena
Zimmer, Christophe
NATURE COMMUNICATIONS, 2018, 9
[14] Optimal Point Spread Function Design for 3D Imaging
Shechtman, Yoav
Sahl, Steffen J.
Backer, Adam S.
Moerner, W. E.
PHYSICAL REVIEW LETTERS, 2014, 113 (13)
[15] Enhanced acoustic streaming effects via sharp-edged 3D microstructures
Harley, William S.
Kolesnik, Kirill
Heath, Daniel E.
Collins, David J.
LAB ON A CHIP, 2024, 24 (06) : 1626 - 1635
[16] Single-frame 3D lensless microscopic imaging via deep learning
Grant-Jacob, James A.
Praeger, Matthew
Eason, Robert W.
Mills, Ben
OPTICS EXPRESS, 2022, 30 (18) : 32621 - 32632
[17] Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited]
Gustavsson, Anna-Karin
Petrov, Petar N.
Moerner, W. E.
OPTICS EXPRESS, 2018, 26 (10): : 13122 - 13147
[18] Advances in 3D single particle localization microscopy
Zhou, Yongzhuang
Handley, Michael
Caries, Guillem
Harvey, Andrew R.
APL PHOTONICS, 2019, 4 (06)
[19] A Detailed Analysis of 3D Subcellular Signal Localization
Pinidiyaarachchi, Amalka
Zieba, Agata
Allalou, Amin
Pardali, Katerina
Wahlby, Carolina
CYTOMETRY PART A, 2009, 75A (04) : 319 - 328
[20] Confocal line scanning of a Bessel beam for fast 3D Imaging
Zhang, P.
Phipps, M. E.
Goodwin, P. M.
Werner, J. H.
OPTICS LETTERS, 2014, 39 (12) : 3682 - 3685

← 1 2 3 4 5 →