D-aspartate oxidase is present in ovaries, eggs and embryos but not in testis of Xenopus laevis

被引：4
作者：
Tedeschi, G
Negri, A
Bernardini, G
Oungre, E
Ceciliani, F
Ronchi, S
机构：
[1] Ist Fisiol Vet & Biochim, I-20133 Milan, Italy
[2] Dipartimento Biol Strutturale & Funz, I-21100 Varese, Italy
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1999年 / 124卷 / 04期
关键词：
D-aspartic acid; D-aspartate oxidase; D-aspartate oxidase antibodies; 2D electrophoresis; Xenopus laevis; gender dependence; testis; ovaries; development;
D O I：
10.1016/S0305-0491(99)00141-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
D-aspartate oxidase (DASPO) is an FAD dependent flavoprotein which catalyzes the oxidative deamination of D-aspartate using oxygen as electron acceptor. D-aspartate and DASPO are supposed to be involved in the regulation of the central nervous system and in the animal development. This manuscript describes for the first time the presence of DASPO in Xenopus laevis fertilized eggs and embryos and suggests a different tissue distribution of this enzyme in adult male and female animals. In particular, by means of 2D-electrophoresis and affinity purified specific anti-DASPO antibodies, the enzyme was localized in fertilized eggs of X. laevis and in ovaries of adult animals but it was shown to be absent in the testis suggesting a gender specific expression. The protein from Xenopus ovaries has been purified by means of immunoprecipitation and it has M-r of 30 kDa and pi of 8.1. (C) 1999 Elsevier Science Inc. All rights reserved.
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收藏
页码：489 / 494
页数：6
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