DNA methylation changes in cell line from β-lactoglobulin gene targeted fetus

被引：5

作者：

Lin, Li ^{[1
]}

Xu, Weizhuo ^{[2
]}

Dai, Yunping ^{[1
]}

Li, Ning ^{[1
]}

机构：

[1] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China

[2] Shenyang Pharmaceut Univ, Dept Biol Sci & Biopharmaceut, Shenyang 110016, Liaoning Prov, Peoples R China

来源：

ANIMAL REPRODUCTION SCIENCE | 2009年 / 112卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

DNA methylation; Gene targeting; Cloning; MAMMALIAN DEVELOPMENT; EMBRYOS; EXPRESSION; GENOME;

D O I：

10.1016/j.anireprosci.2008.05.072

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The gene targeting combined somatic cell nuclear transfer is very useful in agriculture and medicine. Epigenetic modification of DNA by methylation is significant in regulating gene expression during mammalian development. During gene targeting, epigenetic status of donor cell nuclei may be changed in a series of processes, including homologous recombination, cell selection and cloning. We examined DNA methylation of six genes (beta-actin, VEGF, oct4, TERT H19 and Igf2) and a repetitive sequence art2 in blg(+/-) cell line from beta-lactoglobulin (BLG) gene targeted fetus and the cells used for BLG gene targeting serve as control. The results demonstrated that the widespread changes of DNA methylation were found in blg(+/-) cell line. But the degree of variation was different. DNA methylation of VEGF in blg(+/-) was noticeably decreased. These observations suggest that DNA methylation variations may impact gene expression and finally induce abnormalities and lethality in later developmental stages. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：402 / 408

页数：7

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