Oxygen increases the steady-state level of nitrate in denitrifying cells of Paracoccus denitrificans

被引：0
|
作者：
Kucera, I
Kaplan, P
Zeman, A
机构：
[1] MASARYK UNIV,FAC SCI,DEPT INORGAN CHEM,CR-61137 BRNO,CZECH REPUBLIC
[2] MASARYK UNIV,FAC MED,DEPT BIOCHEM,CR-66243 BRNO,CZECH REPUBLIC
来源：
FEMS MICROBIOLOGY LETTERS | 1996年 / 145卷 / 02期
关键词：
denitrification; nitrate; oxygen; transport; inhibition; Paracoccus denitrificans;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The levels of nitrate in denitrifying cells of Paracoccus denitrificans were determined by centrifugation through silicone oil into phosphoric acid and ion-exchange HPLC analysis of the cell lysates, using [C-14]sucrose to correct for the trapped external medium. Introduction of oxygen brought about a significant upward shift in the intracellular nitrate concentration. This result calls into question the current thinking that oxygen blocks nitrate respiration primarily due to the inhibition of nitrate transport into the cell.
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页码：163 / 166
页数：4
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