Tetraphenylethene-based Zn complexes for the highly sensitive detection of single-stranded DNA

被引：13
作者：
Wang, Sheng [1 ]
Zhu, Zece [1 ]
Wei, Danqing [1 ]
Yang, Chuluo [1 ]
机构：
[1] Wuhan Univ, Dept Chem, Hubei Collaborat Innovat Ctr Adv Organ Chem Mat, Hubei Key Lab Organ & Polymer Optoelect Mat, Wuhan 430072, Peoples R China
来源：
JOURNAL OF MATERIALS CHEMISTRY C | 2015年 / 3卷 / 45期
关键词：
AGGREGATION-INDUCED EMISSION; MOLECULAR LIGHT SWITCH; AMINO-MODIFIED TETRAPHENYLETHENE; AQUEOUS-SOLUTION; SELECTIVE DETECTION; CONJUGATED POLYMERS; ETHIDIUM-BROMIDE; NUCLEIC-ACIDS; FLUORESCENCE; RECOGNITION;
D O I：
10.1039/c5tc02869h
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Metal-ligand coordination interactions were utilized to develop tetraphenylethene-based DNA probes. Z-and E-TPE derivatives based on Zn2+-DPA (dipicolylamine) units, namely Z-TPE2Zn and E-TPE2Zn, were tested, respectively, to oligonucleotides with different lengths and different sequences. TPE2Zn exhibited a high fluorescence enhancement when detecting ssDNA as short as 5 nt. The fluorescence intensity of Z-TPE2Zn could increase to more than 100-fold of its initial value when enough DNA was added. This fluorescent on/off ratio was much higher than that of the classical DNA probe of ethidium bromide (EB). Absorption titration experiments indicated that thymine and guanine could coordinate with the Zn2+-DPA unit more effectively than adenine and cytosine. These experiments might aid the design of metal complex-based DNA probes with high sensitivity and selectivity.
引用
收藏
页码：11902 / 11906
页数：5
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