Tetraphenylethene-based Zn complexes for the highly sensitive detection of single-stranded DNA

被引：13

作者：

Wang, Sheng ^{[1
]}

Zhu, Zece ^{[1
]}

Wei, Danqing ^{[1
]}

Yang, Chuluo ^{[1
]}

机构：

[1] Wuhan Univ, Dept Chem, Hubei Collaborat Innovat Ctr Adv Organ Chem Mat, Hubei Key Lab Organ & Polymer Optoelect Mat, Wuhan 430072, Peoples R China

来源：

JOURNAL OF MATERIALS CHEMISTRY C | 2015年 / 3卷 / 45期

关键词：

AGGREGATION-INDUCED EMISSION; MOLECULAR LIGHT SWITCH; AMINO-MODIFIED TETRAPHENYLETHENE; AQUEOUS-SOLUTION; SELECTIVE DETECTION; CONJUGATED POLYMERS; ETHIDIUM-BROMIDE; NUCLEIC-ACIDS; FLUORESCENCE; RECOGNITION;

D O I：

10.1039/c5tc02869h

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Metal-ligand coordination interactions were utilized to develop tetraphenylethene-based DNA probes. Z-and E-TPE derivatives based on Zn2+-DPA (dipicolylamine) units, namely Z-TPE2Zn and E-TPE2Zn, were tested, respectively, to oligonucleotides with different lengths and different sequences. TPE2Zn exhibited a high fluorescence enhancement when detecting ssDNA as short as 5 nt. The fluorescence intensity of Z-TPE2Zn could increase to more than 100-fold of its initial value when enough DNA was added. This fluorescent on/off ratio was much higher than that of the classical DNA probe of ethidium bromide (EB). Absorption titration experiments indicated that thymine and guanine could coordinate with the Zn2+-DPA unit more effectively than adenine and cytosine. These experiments might aid the design of metal complex-based DNA probes with high sensitivity and selectivity.

引用

页码：11902 / 11906

页数：5

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