Reverse hydrolysis catalysed by beta-N-acetylhexosaminidase from Aspergillus oryzae

被引：38
|
作者：
Rajnochova, E [1 ]
Dvorakova, J [1 ]
Hunkova, Z [1 ]
Kren, V [1 ]
机构：
[1] ACAD SCI CZECH REPUBL,INST MICROBIOL,LAB BIOTRANSFORMAT,CR-14220 PRAGUE 4,CZECH REPUBLIC
来源：
BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 09期
关键词：
D O I：
10.1023/A:1018385520155
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
beta-N-Acetylhexosaminidase from Aspergillus oryzae catalyzes the reverse glycosylation of N-acetylglucosamine (1.5 M) to GlcNAcp beta 1-->6GlcNAcp in 14% yield. Addition of salts [LiCl, (NH4)(2)SO4 0.1-1 M] lowers the water activity in the system thus increasing the yield of the thermodynamically controlled reaction. Benzylalcohol as acceptor gives under the same conditions benzyl O-beta-D-2-acetamido-2-deoxy-glucopyranoside.
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页码：869 / 872
页数：4
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