Reverse hydrolysis catalysed by beta-N-acetylhexosaminidase from Aspergillus oryzae

被引：38

作者：

Rajnochova, E ^{[1
]}

Dvorakova, J ^{[1
]}

Hunkova, Z ^{[1
]}

Kren, V ^{[1
]}

机构：

[1] ACAD SCI CZECH REPUBL,INST MICROBIOL,LAB BIOTRANSFORMAT,CR-14220 PRAGUE 4,CZECH REPUBLIC

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 09期

关键词：

D O I：

10.1023/A:1018385520155

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

beta-N-Acetylhexosaminidase from Aspergillus oryzae catalyzes the reverse glycosylation of N-acetylglucosamine (1.5 M) to GlcNAcp beta 1-->6GlcNAcp in 14% yield. Addition of salts [LiCl, (NH4)(2)SO4 0.1-1 M] lowers the water activity in the system thus increasing the yield of the thermodynamically controlled reaction. Benzylalcohol as acceptor gives under the same conditions benzyl O-beta-D-2-acetamido-2-deoxy-glucopyranoside.

引用

页码：869 / 872

页数：4

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