Improved Longevity of Hematopoiesis in Long-term Bone Marrow Cultures and Reduced Irradiation-induced Pulmonary Fibrosis in Toll-like Receptor-4 Deletion Recombinant-Negative Mice

被引：0

作者：

Rhieu, Byung Han ^{[1
]}

Epperly, Michael W. ^{[1
]}

Cao, Shaonan ^{[1
]}

Goff, Julie ^{[1
]}

Shields, Donna ^{[1
]}

Franicola, Darcy ^{[1
]}

Wang, Hong ^{[1
]}

Greenberger, Joel S. ^{[1
]}

机构：

[1] Univ Pittsburgh, Inst Canc, Dept Radiat Oncol, Pittsburgh, PA 15232 USA

来源：

IN VIVO | 2014年 / 28卷 / 04期

关键词：

Hematopoiesis; long-term bone marrow cultures; TLR4; STROMAL CELLS; INJURY; LINE;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aim: We measured long-term hematopoiesis in continuous bone marrow cultures derived from Toll-like receptor-4 (Tlr4(-/-))(C57BL/6J) mice. Materials and Methods: We measured hematopoiesis in vitro over 27 weeks in long-term bone marrow cultures from Tlr4(-/-) and control mice, and irradiation-induced pulmonary fibrosis in mice irradiated to 20 Gy to the thorax. Results: There was a significant increase in the duration of hematopoiesis in long-term bone marrow cultures from Tlr4(-/-) mice in production of total non-adherent cells and day 7 and day 14 multi-lineage colony-forming cells. The histology of bone marrow hematopoietic and stromal cell lines was indistinguishable between different mouse strains. There was no detectable late irradiation pulmonary fibrosis in Tlr4(-/-) mice. Conclusion: Homozygous deletion of both alleles of Tlr4, encoding for an inflammatory mediator receptor, improves the duration of hematopoiesis in vitro and reduces irradiation-induced lung fibrosis.

引用

页码：441 / 448

页数：8

共 7 条

[1] Increased Hematopoiesis in Long-term Bone Marrow Cultures and Reduced Irradiation-induced Pulmonary Fibrosis in Von Willebrand Factor Homologous Deletion Recombinant Mice
Rhieu, Byung Han
Epperly, Michael W.
Cao, Shaonan
Franicola, Darcy
Shields, Donna
Goff, Julie
Wang, Hong
Greenberger, Joel S.
IN VIVO, 2014, 28 (04): : 449 - 456
[2] Reduced Radiation Pulmonary Fibrosis in Toll-Like Receptor-4 (TLR4) Deletion Recombinant Negative Mice
Epperly, M.
Rhieu, B.
Cao, S.
Goff, J.
Shields, D.
Franicola, D.
Wang, H.
Greenberger, J. S.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 2014, 90 : S810 - S811
[3] Reduced Radiation Pulmonary Fibrosis in Toll-Like Receptor-4 (TLR4) Deletion Recombinant Negative Mice
Epperly, M.
Rhieu, B.
Cao, S.
Goff, J.
Shields, D.
Franicola, D.
Wang, H.
Greenberger, J. S.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 2014, 90 : S150 - S150
[4] Correlation of ionizing irradiation-induced late pulmonary fibrosis with long-term bone marrow culture fibroblast progenitor cell biology in mice homozygous deletion recombinant negative for endothelial cell adhesion molecules
Epperly, MW
Guo, HL
Shields, D
Zhang, XC
Greenberger, JS
IN VIVO, 2004, 18 (01): : 1 - 14
[5] Bone marrow from CD18-/- (MAC-1-/-) homozygous deletion recombinant negative mice demonstrates increased longevity in long-term bone marrow culture and decreased contribution to irradiation pulmonary damage
Epperly, Michael W.
Shields, Donna
Niu, Yunyun
Carlos, Timothy
Greenberger, Joel S.
IN VIVO, 2006, 20 (04): : 431 - 438
[6] Radioresistance of hematopoietic progenitors and increased longevity of long-term bone marrow cultures (LTBMCs) from mitochondrial/neuronal nitric oxide synthase (NOS1) homologous deletion recombinant negative (-/-) mice
Cao, S.
Epperly, M. W.
Zhang, X.
Niu, Y.
Greenberger, E. E.
Greenberger, J. S.
EXPERIMENTAL HEMATOLOGY, 2006, 34 (09) : 83 - 83
[7] Neuronal/mitochondrial nitric oxide synthase homologous deletion recombinant negative mice (NOS1-/-) long-term bone marrow cultures (LTBMCs) demonstrate increased longevity and radioresistance of derived cell lines.
Epperly, Michael W.
Greenberger, Joel S.
Cao, Shaonan
Franicola, Darcy
Shen, Hongmei
Greenberger, Emily E.
Epperly, Laura D.
Zhang, Xicheng
BLOOD, 2006, 108 (11) : 395A - 396A

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