Novel poly(vinyl alcohol)-degrading enzyme and the degradation mechanism

被引：116

作者：

Matsumura, S ^{[1
]}

Tomizawa, N ^{[1
]}

Toki, A ^{[1
]}

Nishikawa, K ^{[1
]}

Toshima, K ^{[1
]}

机构：

[1] Keio Univ, Fac Sci & Technol, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan

来源：

MACROMOLECULES | 1999年 / 32卷 / 23期

关键词：

D O I：

10.1021/ma990727b

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A novel poly(vinyl alcohol) (PVA) degradation pathway was demonstrated such that the hydroxy group of PVA was first dehydrogenated into the corresponding carbonyl group to form the beta-hydroxy ketone moiety which was followed by the aldolase-type cleavage to produce the methyl ketone and the aldehyde terminals by the PVA-assimilating strain, Alcaligenes faecalis KK314. Both the biodegradation steps of dehydrogenation and subsequent aldolase-type cleavage were catalyzed by the same protein having 67 kDa as the holoenzyme and apoenzyme of PVA dehydrogenase, respectively.

引用

页码：7753 / 7761

页数：9

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