Engineering RGB color vision into Escherichia coli

被引：146

作者：

Fernandez-Rodriguez, Jesus ^{[1
]}

Moser, Felix ^{[1
]}

Song, Miryoung ^{[1
]}

Voigt, Christopher A. ^{[1
]}

机构：

[1] MIT, Dept Biol Engn, Synthet Biol Ctr, 77 Massachusetts Ave, Cambridge, MA 02139 USA

来源：

NATURE CHEMICAL BIOLOGY | 2017年 / 13卷 / 07期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

REGULATED GENE-EXPRESSION; LIGHT; SYSTEMS; PROTEIN; PHYTOCHROMES; OPTOGENETICS; BACTERIA; PLATFORM; DESIGN; CELLS;

D O I：

10.1038/nchembio.2390

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Optogenetic tools use colored light to rapidly control gene expression in space and time. We designed a genetically encoded system that gives Escherichia coli the ability to distinguish between red, green, and blue (RGB) light and respond by changing gene expression. We use this system to produce 'color photographs' on bacterial culture plates by controlling pigment production and to redirect metabolic flux by expressing CRISPRi guide RNAs.

引用

页码：706 / +

页数：5

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