Influence of copper on the interaction between cytochrome c and sulfite in vitro

被引:1
|
作者
Wang, Jinsheng
Guo, Dongsheng [1 ]
Yuan, Xiaoying
机构
[1] Shanxi Univ, Coll Environm Resources, Taiyuan 030006, Peoples R China
[2] Shanxi Univ, Coll Chem & Chem Engn, Taiyuan 030006, Peoples R China
关键词
copper; cytochrome c; sulfite; fluorescence spectrometry; ultraviolet absorption spectrometry;
D O I
10.1002/jbt.20143
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The quantitative influence of copper on the interaction between cytochrome c and sulfite, which is a derivate of sulfur dioxide in the human body, has been studied by fluorescence spectrometry and ultraviolet absorption spectrometry in vitro. The results indicate that copper may intensely combine with protein component and ferroporphyrin component in cytochrome c at the concentration of 0.1 mM, and the respective association constants (K-A) are 3.77 x 10(4) L mol(-1) and 9.38 x 10(3) L mol(-1). Sulfite has little interaction with the protein component in cytochrome c (K-A = 0.094 L mol(-1)), at either low concentrations or relatively high concentrations (< 0.15 M). However, it can react with the ferroporphyrin component in cytochrome c (K-A = 4.297 L mol(-1)). After copper is added to the sulfite-cytochrome c binary systems, the reaction between sulfite and the protein component in cytochrome c is obviously strengthened at a low concentration (K-A = 7.289 L mol(-1)), while the addition of copper merely has a little effect on the interaction between sulfite and the ferroporphyrin component in cytochrome c. (c) 2006 Wiley Periodicals, Inc.
引用
收藏
页码:255 / 258
页数:4
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