Proton motive force-dependent efflux of tetracycline in clinical isolates of Helicobacter pylori

被引：36
作者：
Anoushiravani, Mona [1 ]
Falsafi, Tahereh [1 ]
Niknam, Vahid [2 ]
机构：
[1] Alzahra Univ, Dept Biol, Tehran, Iran
[2] Univ Tehran, Dept Biol, Tehran, Iran
来源：
JOURNAL OF MEDICAL MICROBIOLOGY | 2009年 / 58卷 / 10期
关键词：
RIBOSOMAL-RNA MUTATIONS; ANTIBIOTIC-RESISTANCE; SUSCEPTIBILITY; METRONIDAZOLE; CLARITHROMYCIN; AMOXICILLIN; INFECTION; CHILDREN; GENES;
D O I：
10.1099/jmm.0.010876-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The aim of this study was to evaluate the role of proton motive force (PMF)-dependent efflux in resistance of Helicobacter pylori to tetracycline (Tet). Tet MIC was determined by agar dilution in the presence and absence of carbonyl cyanide m-chlorophenylhydrazone (CCCP), an inhibitor of PMF. Antibiotic accumulation was conducted in the presence or absence of CCCP and the fluorescence of the accumulated antibiotic was measured by spectrofluorometry. In the presence of CCCP, antibiotic accumulation was increased by 2-17-fold in 17/20 Tet isolates and by 31 10-fold in four of five high-level-resistant mutants. Correlation was observed between this increase and diminution of MIC with CCCP. PMF-dependent efflux mechanisms therefore appear to play an important role in the resistance of clinical isolates of H. pylori to Tet.
引用
收藏
页码：1309 / 1313
页数：5
相关论文
共 25 条
[1]   Multilaboratory comparison of proficiencies in susceptibility testing of Helicobacter pylori and correlation between agar dilution and E test methods [J].
Best, LM ;
Haldane, DJM ;
Keelan, M ;
Taylor, DE ;
Thomson, ABR ;
Loo, V ;
Fallone, CA ;
Lyn, P ;
Smaill, FM ;
Hunt, R ;
Gaudreau, C ;
Kennedy, J ;
Alfa, M ;
Pelletier, R ;
van Zanten, SJOV .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (10) :3138-3144
[2]   Helicobacter pylori uptake and efflux:: Basis for intrinsic susceptibility to antibiotics in vitro [J].
Bina, JE ;
Alm, RA ;
Uria-Nickelsen, M ;
Thomas, SR ;
Trust, TJ ;
Hancock, REW .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (02) :248-254
[3]   Tetracycline antibiotics: Mode of action, applications, molecular biology, and epidemiology of bacterial resistance [J].
Chopra, I ;
Roberts, M .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2001, 65 (02) :232-+
[4]   Emergence of tetracycline resistance in Helicobacter pylori:: Multiple mutational changes in 16S ribosomal DNA and other genetic loci [J].
Dailidiene, D ;
Bertoli, MT ;
Miciuleviciene, J ;
Mukhopadhyay, AK ;
Dailide, G ;
Pascasio, MA ;
Kupcinskas, L ;
Berg, DE .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (12) :3940-3946
[5]   Application of Stool-PCR test for diagnosis of Helicobacter pylori infection in children [J].
Falsafi, Tahereh ;
Favaecli, Raha ;
Mahjoub, Fatemeh ;
Najafi, Mehri .
WORLD JOURNAL OF GASTROENTEROLOGY, 2009, 15 (04) :484-488
[6]   Culture of Helicobacter pylori from stool samples in children [J].
Falsafi, Tahereh ;
Valizadeh, Nargess ;
Najafi, Mehri ;
Ehsani, Azadeh ;
Khani, Afsaneh ;
Landarani, Zahra ;
Falahi, Zahra .
CANADIAN JOURNAL OF MICROBIOLOGY, 2007, 53 (03) :411-416
[7]   Real-time PCR screening for 16S rRNA mutations associated with resistance to tetracycline in Helicobacter pylori [J].
Glocker, E ;
Berning, M ;
Gerrits, MM ;
Kusters, JG ;
Kist, M .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (08) :3166-3170
[8]   Analysis of metronidazole, clarithromycin and tetracycline resistance of Helicobacter pylori isolates from Korea [J].
Kim, JJ ;
Reddy, R ;
Lee, M ;
Kim, JG ;
El-Zaatari, FAK ;
Osato, MS ;
Graham, DY ;
Kwon, DH .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2001, 47 (04) :459-461
[9]   Recommendations for susceptibility tests on fastidious organisms and those requiring special handling [J].
King, A .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2001, 48 :77-80
[10]   Compound efflux in Helicobacter pylori [J].
Kutschke, A ;
de Jonge, BLM .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (07) :3009-3010
123