Optimisation of microwave-assisted extraction of prune (Prunus domestica) antioxidants by response surface methodology

被引:18
作者
Haddadi-Guemghar, Hayate [1 ]
Janel, Nathalie [2 ]
Dairou, Julien [2 ]
Remini, Hocine [1 ]
Madani, Khodir [1 ]
机构
[1] Univ Bejaia, Fac Sci Nat & Vie, Lab Biomath Biophys Biochim & Scientometrie, Bejaia 06000, Algeria
[2] Univ Paris Diderot, Sorbonne Paris Cite, Unite Biol Fonct & Adaptat BFA UMR CNRS 8251, Bioprofiler Facil, F-75205 Paris, France
关键词
Antioxidant activity; epicatechin gallate; microwave-assisted extraction; prune; response surface methodology; total phenols; PHENOLIC-COMPOUNDS; LEAVES; FRUITS;
D O I
10.1111/ijfs.12526
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Optimal conditions for microwave-assisted extraction (MAE) of total phenols (TP), epicatechin gallate and antioxidant activity from prune (Prunus domestica), rejected in transformation process of plum to prune, were determined by response surface methodology. The central composite design was used to study the effects of three independent variables: microwave power, irradiation time and solvent polarity on the TP, epicatechin gallate and antioxidant activity. Epicatechin gallate was identified as a major phenolic compound in prune extract by RP-HPLC. Microwave power and irradiation time significantly affected all responses (P < 0.01). The highest TP (598.89 mg GAE/100 g prune) was obtained using water as an extraction solvent at 500 W, during 115 s. However, the optimal conditions for epicatechin gallate extraction were ethanol 80%, 435 W and 120 s. MAE is more efficient than conventional extraction method to obtain TP from prune. The experimental values were reasonably close to the predicted values confirming the validity of the predicted models.
引用
收藏
页码:2158 / 2166
页数:9
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