Diagnostic PCR: Comparative sensitivity of four probe chemistries

被引：38

作者：

Josefsen, Mathilde H. ^{[1
]}

Lofstrom, Charlotta ^{[1
]}

Sommer, Helle M. ^{[1
]}

Hoorfar, Jeffrey ^{[1
]}

机构：

[1] Tech Univ Denmark, Natl Food Inst, DK-2860 Soborg, Denmark

来源：

MOLECULAR AND CELLULAR PROBES | 2009年 / 23卷 / 3-4期

关键词：

Real-time PCR; Probe; LNA; MGB; Scorpion; TaqMan; QUANTITATIVE PCR; DNA PROBES; PRIMERS; TOOL; LNA;

D O I：

10.1016/j.mcp.2009.02.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three probe chemistries: locked nucleic acid (LNA), minor groove binder (MGB) and Scorpion were compared with a TaqMan probe in a validated real-time PCR assay for detection of food-borne thermotolerant Campylobacter. The LNA probe produced significantly lower Ct-values and a higher proportion of positive PCR responses analyzing less than 150 DNA copies than the TaqMan probe. Choice of probe chemistry clearly has an impact on the sensitivity of PCR assays, and should be considered in an optimization strategy. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：201 / 203

页数：3

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