Microsphere-based real-time calcium sensing

被引：60

作者：

Sanchez-Martin, Rosario M.

Cuttle, Matt

Mittoo, Stifun

Bradley, Mark

机构：

[1] Univ Edinburgh, Sch Chem, Edinburgh EH9 3JJ, Midlothian, Scotland

[2] Univ Southampton, Sch Biol Sci, Southampton SO16 7PX, Hants, England

[3] Activotec, Southampton SO16 7PX, Hants, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 33期

关键词：

biosensors; fluorescent probes; in vitro sensing; microspheres;

D O I：

10.1002/anie.200601242

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sensing within the cell: Microspheres (2 μm) covalently loaded with calcium sensors can be delivered efficiently into living cells to analyze the intracellular changes in the concentration of Ca2+ ions in real time. The figure shows the real-time ratiometric fluorescent analysis (400/475 nm ratio) of the release of Ca2+ ions in cells loaded with the microspheres. (Graph Presented). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：5472 / 5474

页数：3

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