Molecular characterization of Escherichia coli O157:H7 hide contamination routes:: Feedlot to harvest

被引:23
作者
Childs, K. D.
Simpson, C. A.
Warren-Serna, W.
Bellenger, G.
Centrella, B.
Bowling, R. A.
Ruby, J.
Stefanek, J.
Vote, D. J.
Choat, T.
Scanga, J. A.
Sofos, J. N.
Smith, G. C.
Belk, K. E. [1 ]
机构
[1] Colorado State Univ, Dept Anim Sci, Ctr Red Meat Safety, Ft Collins, CO 80523 USA
[2] Smithfield Beef Grp, Green Bay, WI 54305 USA
[3] Food Safety Net Serv, San Antonio, TX 78216 USA
关键词
D O I
10.4315/0362-028X-69.6.1240
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This study was conducted to identify the origin of Escherichia coli O157:147 contamination on steer hides at the time of harvest. Samples were collected from the feedlot, transport trailers, and packing plant holding pens and from the colons and hides of feedlot steers. A total of 50 hide samples were positive for E. coli O157:H7 in two geographical locations: the Midwest (25 positive hides) and Southwest (25 positive hides). Hide samples were screened, and the presence of E. coli O157: H7 was confirmed. E. coli O157:H7 isolates were fingerprinted by pulsed-field gel electrophoresis and subjected to multiplex PCR procedures for amplification of E. coli O15:H7 genes stx(1), stx(2), eaeA, fliC, rfbE(O157), and hlyA. Feedlot water trough, pen floor, feed bunk, loading chute, truck trailer side wall and floor, packing plant holding pen floor and side rail, and packing plant cattle drinking water samples were positive for E. coli O15:H7. Pulsed-field gel electrophoresis banding patterns were analyzed after classifying isolates according to the marker genes present and according to packing plant. In this study, hide samples positive for E. coli O157:H7 were traced to other E. coli O157:H7-positive hide, colon, feedlot pen floor fecal, packing plant holding pen drinking water, and transport trailer side wall samples. Links were found between packing plant side rails, feedlot loading chutes, and feedlot pens and between truck trailer, different feedlots, and colons of multiple cattle. This study is the first in which genotypic matches have been made between E. coli 015:H7 isolates obtained from transport trailer side walls and those from cattle hide samples within the packing plant.
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收藏
页码:1240 / 1247
页数:8
相关论文
共 27 条
[1]   Seasonal prevalence of Shiga toxin-producing Escherichia coli, including O157:H7 and non-O157 serotypes, and Salmonella in commercial beef processing plants [J].
Barkocy-Gallagher, GA ;
Arthur, TM ;
Rivera-Betancourt, M ;
Nou, XW ;
Shackelford, SD ;
Wheeler, TL ;
Koohmaraie, M .
JOURNAL OF FOOD PROTECTION, 2003, 66 (11) :1978-1986
[2]   Development of methods for the recovery of Escherichia coli O157:H7 and Salmonella from beef carcass sponge samples and bovine fecal and hide samples [J].
Barkocy-Gallagher, GA ;
Berry, ED ;
Rivera-Betancourt, M ;
Arthur, TM ;
Nou, XW ;
Koohmaraie, M .
JOURNAL OF FOOD PROTECTION, 2002, 65 (10) :1527-1534
[3]   Genotypic analyses of Escherichia coli O157:H7 and O157 nonmotile isolates recovered from beef cattle and carcasses at processing plants in the midwestern states of the United States [J].
Barkocy-Gallagher, GA ;
Arthur, TM ;
Siragusa, GR ;
Keen, JE ;
Elder, RO ;
Laegreid, WW ;
Koohmaraie, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (09) :3810-3818
[4]   Distribution and sources of microbial contamination on beef carcasses [J].
Bell, RG .
JOURNAL OF APPLIED MICROBIOLOGY, 1997, 82 (03) :292-300
[5]   CATTLE AS A POSSIBLE SOURCE OF VEROCYTOTOXIN-PRODUCING ESCHERICHIA-COLI-O157 INFECTIONS IN MAN [J].
CHAPMAN, PA ;
WRIGHT, DJ ;
NORMAN, P ;
FOX, J ;
CRICK, E .
EPIDEMIOLOGY AND INFECTION, 1993, 111 (03) :439-447
[6]   Evaluation of pulsed-field gel electrophoresis as a tool for determining the degree of genetic relatedness between strains of Escherichia coli O157:H7 [J].
Davis, MA ;
Hancock, DD ;
Besser, TE ;
Call, DR .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (05) :1843-1849
[7]   Prevalence of and risk factors for Escherichia coli O157 in market-ready beef cattle from 12 US feedlots [J].
Dewell, GA ;
Ransom, JR ;
Dewell, RD ;
McCurdy, K ;
Gardner, IA ;
Hill, AE ;
Sofos, JN ;
Belk, KE ;
Smith, GC ;
Salmanl, MD .
FOODBORNE PATHOGENS AND DISEASE, 2005, 2 (01) :70-76
[8]   Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing [J].
Elder, RO ;
Keen, JE ;
Siragusa, GR ;
Barkocy-Gallagher, GA ;
Koohmaraie, M ;
Laegreid, WW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (07) :2999-3003
[9]   An investigation of Escherichia coli O157 contamination of cattle during slaughter at an abattoir [J].
Fegan, N ;
Higgs, G ;
Vanderlinde, P ;
Desmarchelier, P .
JOURNAL OF FOOD PROTECTION, 2005, 68 (03) :451-457
[10]   Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains [J].
Gannon, VPJ ;
DSouza, S ;
Graham, T ;
King, RK ;
Rahn, K ;
Read, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (03) :656-662