Protein-Patterning on Functionalized, Non-Biofouling Poly[N-acryloxysuccinimide-co-oligo(ethylene glycol) methyl ether methacrylate] Film-Coated PET Surfaces

被引：9
作者：
Han, Gyeongyeop [1 ,2 ]
Kim, Yoonyoung [3 ]
Kang, Kyungtae [3 ]
Lee, Bong Soo [4 ]
Lee, Jungkyu K. [1 ,2 ]
机构：
[1] Kyungpook Natl Univ, Dept Chem, Daegu 41566, South Korea
[2] Kyungpook Natl Univ, Green Nano Mat Res Ctr, Daegu 41566, South Korea
[3] Kyung Hee Univ, Dept Appl Chem, Gyeonggi 17104, South Korea
[4] Korea Adv Inst Sci & Technol, Dept Chem, Ctr Cell Encapsulat Res, Daejeon 34141, South Korea
来源：
MACROMOLECULAR RESEARCH | 2018年 / 26卷 / 03期
基金：
新加坡国家研究基金会;
关键词：
organic polymer substrate; copolymer coating; biopatterning; surface-initiated controlled radical polymerization; photochemical reaction; CYCLIC OLEFIN COPOLYMER; VASCULAR PROSTHESES; IMMOBILIZATION; BIOMOLECULES; INITIATOR; CELLS; BIOCOMPATIBILITY; POLYMERIZATION; METHACRYLATE); GENERATION;
D O I：
10.1007/s13233-018-6035-y
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
We successfully fabricated poly(ethylene terephthalate) (PET) surfaces through a perfluoroaryl azide-based photochemical reaction, and subsequently formed an intrinsically activated, non-biofouling poly[N-acryloxysuccinimide-co-oligo(ethylene glycol) methyl ether methacrylate] on the surface through surface-initiated, controlled radical polymerization. The grafted copolymer film on PET facilely generated a protein pattern using a microcontact printing technique without employing both an activation step to introduce an active functional group (e.g., succinimidyl ester) and a passivation process for minimizing non-specific adsorption. Consequently, we characterized the functionalized PET surfaces by using various methods including contact angle measurement, X-ray photoelectron spectroscopy (XPS), scanning probe microscopy (SPM), field-emission scanning electron microscopy (FE-SEM). In addition, we evaluated the non-biofouling efficacy of the protein-patterned copolymer film on PET by confocal laser scanning microscopy.
引用
收藏
页码：263 / 269
页数：7
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