Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion

被引：377
作者：
Meissner, M
Schlüter, D
Soldati, D
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Dept Biol Sci, London SW7 2AZ, England
[2] Heidelberg Univ, Klinikum Mannheim, Inst Med Mikrobiol & Hyg, D-68167 Mannheim, Germany
来源：
SCIENCE | 2002年 / 298卷 / 5594期
关键词：
D O I：
10.1126/science.1074553
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Obligate intracellular apicomplexan parasites rely on gliding motion powered by their actomyosin system to disperse throughout tissues and to penetrate host cells. Toxoplasma gondii myosin A has been implicated in this process, but direct proof has been lacking. We designed a genetic screen to generate a tetracycline-inducible transactivator system in T. gondii. The MyoA gene was disrupted in the presence of a second regulatable copy of MyoA. Conditional removal of this myosin caused severe impairment in host cell invasion and parasite spreading in cultured cells, and unambiguously established the pathogenic function of this motor in an animal model.
引用
收藏
页码：837 / 840
页数：4
相关论文
共 17 条
  • [1] Ionophore-resistant mutants of Toxoplasma gondii reveal host cell permeabilization as an early event in egress
    Black, MW
    Arrizabalaga, G
    Boothroyd, JC
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (24) : 9399 - 9408
  • [2] The toxoplasma micronemal protein MIC4 is an adhesin composed of six conserved apple domains
    Brecht, S
    Carruthers, VB
    Ferguson, DJP
    Giddings, OK
    Wang, G
    Jäkle, U
    Harper, JM
    Sibley, LD
    Soldati, D
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (06) : 4119 - 4127
  • [3] Secretion of micronemal proteins is associated with toxoplasma invasion of host cells
    Carruthers, VB
    Giddings, OK
    Sibley, LD
    [J]. CELLULAR MICROBIOLOGY, 1999, 1 (03) : 225 - 235
  • [4] Ethanol and acetaldehyde elevate intracellular [Ca2+] and stimulate microneme discharge in Toxoplasma gondii
    Carruthers, VB
    Moreno, SNJ
    Sibley, LD
    [J]. BIOCHEMICAL JOURNAL, 1999, 342 : 379 - 386
  • [5] Toxoplasma invasion of mammalian cells is powered by the actin cytoskeleton of the parasite
    Dobrowolski, JM
    Sibley, LD
    [J]. CELL, 1996, 84 (06) : 933 - 939
  • [6] TIGHT CONTROL OF GENE-EXPRESSION IN MAMMALIAN-CELLS BY TETRACYCLINE-RESPONSIVE PROMOTERS
    GOSSEN, M
    BUJARD, H
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) : 5547 - 5551
  • [7] Time-lapse video microscopy of gliding motility in Toxoplasma gondii reveals a novel, biphasic mechanism of cell locomotion
    Håkansson, S
    Morisaki, H
    Heuser, J
    Sibley, LD
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (11) : 3539 - 3547
  • [8] A novel class of unconventional myosins from Toxoplasma gondii
    Heintzelman, MB
    Schwartzman, JD
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1997, 271 (01) : 139 - 146
  • [9] Toxoplasma gondii myosin A and its light chain:: a fast, single-headed, plus-end-directed motor
    Herm-Götz, A
    Weiss, S
    Stratmann, R
    Fujita-Becker, S
    Ruff, C
    Meyhöfer, E
    Soldati, T
    Manstein, DJ
    Geeves, MA
    Soldati, D
    [J]. EMBO JOURNAL, 2002, 21 (09) : 2149 - 2158
  • [10] A dibasic motif in the tail of a class XIV apicomplexan myosin is an essential determinant of plasma membrane localization
    Hettmann, C
    Herm, A
    Geiter, A
    Frank, B
    Schwarz, E
    Soldati, T
    Soldati, D
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (04) : 1385 - 1400
12