Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system

被引：44

作者：

Takegawa, Kaoru ^{[1
]}

Tohda, Hideki ^{[2
]}

Sasaki, Mayumi ^{[2
]}

Idiris, Alimjan ^{[2
]}

Ohashi, Takao ^{[1
]}

Mukaiyama, Hiroyuki ^{[1
]}

Giga-Hama, Yuko ^{[3
]}

Kumagai, Hiromichi ^{[3
]}

机构：

[1] Kyushu Univ, Dept Biosci & Biotechnol, Fac Agr, Fukuoka 8128581, Japan

[2] Asahi Glass Co Ltd, ASPEX Div, Kanagawa 2218755, Japan

[3] Asahi Glass Co Ltd, ASPEX Div, Chiyoda Ku, Tokyo 1008405, Japan

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2009年 / 53卷

关键词：

fission yeast (Schizosaccharomyces pombe); gene deletion; glycosylation; heterologous protein production; membrane trafficking; O-MANNOSYLTRANSFERASE FAMILY; N-LINKED OLIGOSACCHARIDES; OUTER CHAIN ELONGATION; CELL-WALL INTEGRITY; SACCHAROMYCES-CEREVISIAE; GLOBAL ANALYSIS; ESSENTIAL GENES; IDENTIFICATION; GLYCOSYLATION; SECRETION;

D O I：

10.1042/BA20090048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc. pombe is considered an attractive host for the production of heterologous proteins. Expression vectors for high-level expression in Sc. pombe have been developed and many foreign proteins have been successfully expressed. However, further improvements in the protein-expressing host systems are still required for the production of heterologous proteins involved in post-translational modification, metabolism and intracellular tracking. This minireview focuses on recent advances in heterologous protein production by use of engineered fission-yeast strains.

引用

页码：227 / 235

页数：9

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