Comparison of three real-time PCR assays for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in young pregnant women

被引:13
作者
Peuchant, Olivia [1 ,2 ,3 ]
de Diego, Sabrina [1 ,2 ]
Le Roy, Chloe [1 ,2 ]
Frantz-Blancpain, Sandrine [4 ]
Hocke, Claude [4 ]
Bebear, Cecile [1 ,2 ,3 ]
de Barbeyrac, Bertille [1 ,2 ,3 ]
机构
[1] Univ Bordeaux, USC EA Infect Humaines Mycoplasmes & Chlamydiae 3, Bordeaux, France
[2] INRA, USC EA Infect Humaines Mycoplasmes & Chlamydiae 3, Bordeaux, France
[3] Ctr Hosp Univ Bordeaux, Lab Bacteriol, Bordeaux, France
[4] Ctr Hosp Univ Bordeaux, Serv Gynecol Obstet, Bordeaux, France
关键词
Chlamydia trachomatis; Neisseria gonorrhoeae; Real-time PCRs; Vaginal swabs; Pregnant women; MYCOPLASMA-GENITALIUM; VAGINAL SWABS; TERMINATION; PREVALENCE;
D O I
10.1016/j.diagmicrobio.2015.09.002
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
We compared 3 commercial real-time PCR assays, the Abbott RealTime CT/NG, the cobas (R) 4800 CT/NG, and the Cepheid Xpert (R) CT/NG, for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in vaginal swabs collected prospectively from pregnant women aged <25 years. The overall agreement among 2 assays ranged from 98.9% to 995% with a kappa score between 0.94 and 0.97 for C. trachomatis. For N. gonorrhoeae, the overall agreement was 100%. All kits allowed prompt and specific results for C. trachomatis and N. gonorrhoeae in young pregnant women. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码:335 / 337
页数:3
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