Comparison of three real-time PCR assays for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in young pregnant women

被引:13
作者
Peuchant, Olivia [1 ,2 ,3 ]
de Diego, Sabrina [1 ,2 ]
Le Roy, Chloe [1 ,2 ]
Frantz-Blancpain, Sandrine [4 ]
Hocke, Claude [4 ]
Bebear, Cecile [1 ,2 ,3 ]
de Barbeyrac, Bertille [1 ,2 ,3 ]
机构
[1] Univ Bordeaux, USC EA Infect Humaines Mycoplasmes & Chlamydiae 3, Bordeaux, France
[2] INRA, USC EA Infect Humaines Mycoplasmes & Chlamydiae 3, Bordeaux, France
[3] Ctr Hosp Univ Bordeaux, Lab Bacteriol, Bordeaux, France
[4] Ctr Hosp Univ Bordeaux, Serv Gynecol Obstet, Bordeaux, France
关键词
Chlamydia trachomatis; Neisseria gonorrhoeae; Real-time PCRs; Vaginal swabs; Pregnant women; MYCOPLASMA-GENITALIUM; VAGINAL SWABS; TERMINATION; PREVALENCE;
D O I
10.1016/j.diagmicrobio.2015.09.002
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
We compared 3 commercial real-time PCR assays, the Abbott RealTime CT/NG, the cobas (R) 4800 CT/NG, and the Cepheid Xpert (R) CT/NG, for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in vaginal swabs collected prospectively from pregnant women aged <25 years. The overall agreement among 2 assays ranged from 98.9% to 995% with a kappa score between 0.94 and 0.97 for C. trachomatis. For N. gonorrhoeae, the overall agreement was 100%. All kits allowed prompt and specific results for C. trachomatis and N. gonorrhoeae in young pregnant women. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码:335 / 337
页数:3
相关论文
共 50 条
[31]   Mother-to-child transmission of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis in HIV-infected pregnant women in South Africa [J].
Peters, Remco P. H. ;
Feucht, Ute D. ;
de Vos, Lindsey ;
Ngwepe, Phuti ;
McIntyre, James A. ;
Klausner, Jeffrey D. ;
Medina-Marino, Andrew .
INTERNATIONAL JOURNAL OF STD & AIDS, 2021, 32 (09) :799-805
[32]   Development a rapid and accurate multiplex real time PCR method for the detection Chlamydia trachomatis and Mycoplasma hominis [J].
Safarkar, Roya ;
Mehrabadi, Jalil Fallah ;
Noormohammadi, Zahra ;
Mirnejad, Reza .
JOURNAL OF CLINICAL LABORATORY ANALYSIS, 2017, 31 (06)
[33]   Are Chlamydia Trachomatis and Neisseria Gonorrhoeae Screenings in Pregnant Women Being Properly Performed? A Single-Center Retrospective Observational Study in Italy [J].
Mondi, Vito ;
Caravetta, Jacopo ;
Paolillo, Piermichele ;
Salce, Nicola ;
Tzialla, Chryssoula ;
Vasapollo, Barbara ;
Valensise, Herbert ;
Bedetta, Manuela ;
Picone, Simonetta .
PATHOGENS, 2024, 13 (07)
[34]   Prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis infection in pregnant adolescent women and its association with pregnancy outcomes [J].
Priti Ghope ;
Subash C Sonkar ;
Kirti Wasnik ;
Pratima Mittal ;
Daman Saluja .
BMC Infectious Diseases, 14 (Suppl 3)
[35]   Comparison of Immunochromatographic (IC) and Real-time Polymerase Chain Reaction (Rt-PCR) Tests for Screening Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma spp. and Mycoplasma spp. in HIV-infected Men [J].
Zarakolu, Pinar ;
Cetik, Sila ;
Inkaya, Ahmet Cagkan ;
Unal, Serhat .
MIKROBIYOLOJI BULTENI, 2019, 53 (01) :37-42
[36]   Development and analytical validation of real-time PCR for the detection ofStreptococcus agalactiaein pregnant women [J].
Escobar, Daniel F. ;
Diaz-Dinamarca, Diego A. ;
Hernandez, Carlos F. ;
Soto, Daniel A. ;
Manzo, Ricardo A. ;
Alarcon, Pedro, I ;
Pinto, Camila H. ;
Bastias, Diego N. ;
Oberg-Bravo, Carolayn N. ;
Rojas, Robert ;
Illanes, Sebastian E. ;
Kalergis, Alexis M. ;
Vasquez, Abel E. .
BMC PREGNANCY AND CHILDBIRTH, 2020, 20 (01)
[37]   Prevalence and co-infection study of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis among symptomatic women using PCR assay [J].
SC Sonkar ;
K Wasnik ;
PK Mishra ;
P Mittal ;
A Kumar ;
J Suri ;
D Saluja .
BMC Infectious Diseases, 14 (Suppl 3)
[38]   Comparison between Abbott m2000 RealTime and Alinity m STI systems for detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium [J].
Herrmann, Bjorn ;
Malm, Karin .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2021, 40 (10) :2217-2220
[39]   Development of a disposable cartridge real-time PCR test for detection of Chlamydia trachomatis directly from urine [J].
Brennan, Desmond ;
O'Connor, Louise ;
Cormican, Martin ;
Kileen, James ;
Clancy, Eoin ;
Smith, Terry J. ;
Galvin, Paul .
SENSORS AND ACTUATORS B-CHEMICAL, 2021, 339
[40]   Pooling of urine samples for molecular detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium as a screening strategy among young adults in Catalonia [J].
Lopez-Corbeto, Evelin ;
Gonzalez, Victoria ;
Lugo, Rossie ;
Rivaya, Belen ;
Casabona, Jordi ;
Matas, Lurdes .
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA, 2020, 38 (02) :65-71