Comparison of three real-time PCR assays for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in young pregnant women

被引：13

作者：

Peuchant, Olivia ^{[1
,2
,3
]}

de Diego, Sabrina ^{[1
,2
]}

Le Roy, Chloe ^{[1
,2
]}

Frantz-Blancpain, Sandrine ^{[4
]}

Hocke, Claude ^{[4
]}

Bebear, Cecile ^{[1
,2
,3
]}

de Barbeyrac, Bertille ^{[1
,2
,3
]}

机构：

[1] Univ Bordeaux, USC EA Infect Humaines Mycoplasmes & Chlamydiae 3, Bordeaux, France

[2] INRA, USC EA Infect Humaines Mycoplasmes & Chlamydiae 3, Bordeaux, France

[3] Ctr Hosp Univ Bordeaux, Lab Bacteriol, Bordeaux, France

[4] Ctr Hosp Univ Bordeaux, Serv Gynecol Obstet, Bordeaux, France

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2015年 / 83卷 / 04期

关键词：

Chlamydia trachomatis; Neisseria gonorrhoeae; Real-time PCRs; Vaginal swabs; Pregnant women; MYCOPLASMA-GENITALIUM; VAGINAL SWABS; TERMINATION; PREVALENCE;

D O I：

10.1016/j.diagmicrobio.2015.09.002

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We compared 3 commercial real-time PCR assays, the Abbott RealTime CT/NG, the cobas (R) 4800 CT/NG, and the Cepheid Xpert (R) CT/NG, for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in vaginal swabs collected prospectively from pregnant women aged <25 years. The overall agreement among 2 assays ranged from 98.9% to 995% with a kappa score between 0.94 and 0.97 for C. trachomatis. For N. gonorrhoeae, the overall agreement was 100%. All kits allowed prompt and specific results for C. trachomatis and N. gonorrhoeae in young pregnant women. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：335 / 337

页数：3

共 50 条

[21] Fabrication and optimization of the multiplex PCR-based oligonucleotide microarray for detection of Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum [J].

Shi, G ;

Wen, SY ;

Chen, SH ;

Wang, SQ .

JOURNAL OF MICROBIOLOGICAL METHODS, 2005, 62 (02) :245-256

[22] Development, validation and testing costs of an in-house real-time PCR assay for the detection of Chlamydia trachomatis [J].

dos Santos, Camila Gurgel ;

Sabido, Meritxell ;

Leturiondo, Andre Luiz ;

Ferreira, Cynthia de Oliveira ;

da Cruz, Thielle Pereira ;

Benzaken, Adele Schwartz .

JOURNAL OF MEDICAL MICROBIOLOGY, 2017, 66 (03) :312-317

[23] Genital and extra-genital Chlamydia trachomatis and Neisseria gonorrhoeae infections in young women attending a Sexually Transmitted Infections (STI) clinic [J].

Foschi, Claudio ;

Zagarrigo, Manuel ;

Belletti, Matteo ;

Marangoni, Antonella ;

Re, Maria Carla ;

Gaspari, Valeria .

NEW MICROBIOLOGICA, 2020, 43 (03) :115-120

[24] Review of use of a new rapid real-time PCR, the Cepheid GeneXpert® (Xpert) CT/NG assay, for Chlamydia trachomatis and Neisseria gonorrhoeae: results for patients while in a clinical setting [J].

Gaydos, Charlotte A. .

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2014, 14 (02) :135-137

[25] Prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae infections among pregnant women and eye colonization of their neonates at birth time, Shiraz, Southern Iran [J].

Bahman Pourabbas ;

Zahra Rezaei ;

Jalal Mardaneh ;

Mozhgan Shahian ;

Abdolvahab Alborzi .

BMC Infectious Diseases, 18

[26] Prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae infections among pregnant women and eye colonization of their neonates at birth time, Shiraz, Southern Iran [J].

Pourabbas, Bahman ;

Rezaei, Zahra ;

Mardaneh, Jalal ;

Shahian, Mozhgan ;

Alborzi, Abdolvahab .

BMC INFECTIOUS DISEASES, 2018, 18

[27] Multiplex TaqMan real-time PCR platform for detection of Neisseria gonorrhoeae with decreased susceptibility to ceftriaxone [J].

Zhao, Lihong ;

Liu, Aihua ;

Li, Ruiying ;

Zhao, Shuping .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2019, 93 (04) :299-304

[28] TaqMan Real-Time Quantitative PCR Assay for Detection of Fluoroquinolone-Resistant Neisseria gonorrhoeae [J].

LiHong Zhao ;

ShuPing Zhao .

Current Microbiology, 2012, 65 :692-695

[29] Multiplex Real-Time PCR Assay for Simultaneous Identification of Neisseria gonorrhoeae and Its Ciprofloxacin Susceptibility Status [J].

Perera, Sumudu R. ;

Khan, Nurul H. ;

Martin, Irene ;

Taheri, Ali ;

Parti, Rajinder P. ;

Levett, Paul N. ;

Horsman, Greg B. ;

Kusalik, Anthony ;

Dillon, Jo-Anne R. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2017, 55 (11) :3201-3209

[30] Development of multiplex real-time quantitative PCR for simultaneous detection of Chlamydia trachomatis and Ureaplasma parvum [J].

Wei, Hong-bo ;

Zou, She-xiao ;

Yang, Xiao-lin ;

Yang, Dai-qin ;

Chen, Xiang-dong .

CLINICAL BIOCHEMISTRY, 2012, 45 (09) :663-667

← 1 2 3 4 5 →