Proposed method for agglutinating antibody titer analysis and its use as indicator of acquired immunity in pacu, Piaractus mesopotamicus

被引：13
作者：
Biller-Takahashi, J. D. [1 ,2 ]
Montassier, H. J. [1 ]
Takahashi, L. S. [3 ]
Urbinati, E. C. [2 ]
机构：
[1] Univ Estadual Paulista UNESP, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, Brazil
[2] CAUNESP, BR-14884900 Jaboticabal, SP, Brazil
[3] Univ Estadual Paulista UNESP, Fac Zootecnia, BR-17900000 Dracena, SP, Brazil
来源：
BRAZILIAN JOURNAL OF BIOLOGY | 2014年 / 74卷 / 01期
关键词：
fish; methodology; immunology; acquire immunology; humoral immunity; LEUKOCYTES RESPIRATORY BURST; CARP CYPRINUS-CARPIO; ONCORHYNCHUS-MYKISS; CHANNEL CATFISH; INNATE IMMUNITY; RAINBOW-TROUT; TELEOST FISH; LABEO-ROHITA; LEVAMISOLE; EFFICACY;
D O I：
10.1590/1519-6984.21312
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Antibody can be assessed by agglutinating antibody titer which is a quantitative measure of circulating antibodies in serum from fish previously immunized. The antibody evaluation has been performed with different fish species, and is considered a reliable method that can be applied to confirm several hypothesis regarding acquired immunity, even in conjunction with precise methods to describe immune mechanisms. In order to provide appropriate analytical methods for future studies on the specific immune system of native fish, the present study standardized on assay to measure the serum agglutinating antibody titer produced after immunization with inactivated A. hydrophila and levamisole administration in pacu. It was possible to determine the agglutinating antibodies titer in a satisfactorily way in pacu immunized with inactive A. hydrophila, and the highest titers were observed on fish fed with levamisole.
引用
收藏
页码：238 / 242
页数：5
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