Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak

被引：839

作者：

Gire, Stephen K. ^{[1
,2
,3
]}

Goba, Augustine

Andersen, Kristian G. ^{[1
,2
]}

Sealfon, Rachel S. G. ^{[2
,4
]}

Park, Daniel J. ^{[2
]}

Kanneh, Lansana ^{[3
]}

Jalloh, Simbirie ^{[3
]}

Momoh, Mambu ^{[3
,5
]}

Fullah, Mohamed ^{[3
,5
]}

Dudas, Gytis ^{[6
]}

Wohl, Shirlee ^{[1
,2
,7
]}

Moses, Lina M. ^{[8
]}

Yozwiak, Nathan L. ^{[1
,2
]}

Winnicki, Sarah ^{[1
,2
]}

Matranga, Christian B. ^{[2
]}

Malboeuf, Christine M. ^{[2
]}

Qu, James ^{[2
]}

Gladden, Adrianne D. ^{[2
]}

Schaffner, Stephen F. ^{[1
,2
]}

Yang, Xiao ^{[2
]}

Jiang, Pan-Pan ^{[1
,2
]}

Nekoui, Mahan ^{[1
,2
]}

Colubri, Andres ^{[1
]}

Coomber, Moinya Ruth ^{[3
]}

Fonnie, Mbalu ^{[3
]}

Moigboi, Alex ^{[3
]}

Gbakie, Michael ^{[3
]}

Kamara, Fatima K. ^{[3
]}

Tucker, Veronica ^{[3
]}

Konuwa, Edwin ^{[3
]}

Saffa, Sidiki ^{[3
]}

Sellu, Josephine ^{[3
]}

Jalloh, Abdul Azziz ^{[3
]}

Kovoma, Alice ^{[3
]}

Koninga, James ^{[3
]}

Mustapha, Ibrahim ^{[3
]}

Kargbo, Kandeh ^{[3
]}

Foday, Momoh ^{[3
]}

Yillah, Mohamed ^{[3
]}

Kanneh, Franklyn ^{[3
]}

Robert, Willie ^{[3
]}

Massally, James L. B. ^{[3
]}

Chapman, Sinead B. ^{[2
]}

Bochicchio, James ^{[2
]}

Murphy, Cheryl ^{[2
]}

Nusbaum, Chad ^{[2
]}

Young, Sarah ^{[2
]}

Birren, BruceW. ^{[2
]}

Grant, Donald S. ^{[3
]}

Scheiffelin, John S. ^{[8
]}

机构：

[1] Harvard Univ, Dept Organism & Evolutionary Biol, Ctr Syst Biol, Cambridge, MA 02138 USA

[2] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA

[3] Kenema Govt Hosp, Kenema, Sierra Leone

[4] MIT, Comp Sci & Artificial Intelligence Lab, Cambridge, MA 02139 USA

[5] Eastern Polytech Coll, Kenema, Sierra Leone

[6] Univ Edinburgh, Inst Evolutionary Biol, Edinburgh EH9 3JT, Midlothian, Scotland

[7] Harvard Univ, Sch Med, Boston, MA 02115 USA

[8] Tulane Univ, Med Ctr, New Orleans, LA 70112 USA

[9] MIT, Dept Biol, Cambridge, MA 02139 USA

[10] Redeemers Univ, Redemption, Ogun State, Nigeria

[11] Univ Sierra Leone, Freetown, Sierra Leone

[12] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA

[13] Univ Edinburgh, Ctr Immun Infect & Evolut, Edinburgh EH9 3JT, Midlothian, Scotland

来源：

SCIENCE | 2014年 / 345卷 / 6202期

基金：

欧洲研究理事会; 英国自然环境研究理事会;

关键词：

PURIFYING SELECTION; HEMORRHAGIC-FEVER; POLYMERASE; RNA;

D O I：

10.1126/science.1259657

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In its largest outbreak, Ebola virus disease is spreading through Guinea, Liberia, Sierra Leone, and Nigeria. We sequenced 99 Ebola virus genomes from 78 patients in Sierra Leone to similar to 2000x coverage. We observed a rapid accumulation of interhost and intrahost genetic variation, allowing us to characterize patterns of viral transmission over the initial weeks of the epidemic. This West African variant likely diverged from central African lineages around 2004, crossed from Guinea to Sierra Leone in May 2014, and has exhibited sustained human-to-human transmission subsequently, with no evidence of additional zoonotic sources. Because many of the mutations alter protein sequences and other biologically meaningful targets, they should be monitored for impact on diagnostics, vaccines, and therapies critical to outbreak response.

引用

页码：1369 / 1372

页数：4

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