Structural analysis of water-soluble glucans from the root of Angelica sinensis (Oliv.) Diels

被引：89

作者：

Cao, Wei

Li, Xiao-Qiang

Liu, Li

Wang, Minchang

Fan, Hui-Ting

Li, Chen

Lv, Zhengguang

Wang, Xiaojing

Mei, Qibing ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Sch Pharm, Dept Pharmacol, Xian 710032, Peoples R China

[2] Xian Modern Chem Res Inst, Xian 710065, Peoples R China

[3] E China Univ Sci & Technol, Sch Chem Engn, Shanghai 200237, Peoples R China

来源：

CARBOHYDRATE RESEARCH | 2006年 / 341卷 / 11期

关键词：

Angelica sinensis; structure; polysaccharide; glucan;

D O I：

10.1016/j.carres.2006.04.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two water-soluble glucans (designated APS-1cI and APS-1cII) were extracted from the roots of Angelica sinensis (Oliv.) Diels and further purified by anion-exchange and gel-filtration chromatography. Their molecular weights were determined to be 1.7 x 10(5) and 3.9 x 10(4) Da, respectively. The structures of the purified glucans were investigated by a combination of chemical and instrumental analysis, such as methylation analysis, periodate oxidation, GC-MS, as well as FTIR and NMR spectroscopy (H-1, C-13, H-H COSY, HSQC, HMBC, TOCSY and NOESY). The data obtained indicated that APS-1cI was a linear alpha-glucan composed of only (1 -> 6)-alpha-D-G1cp, and APS-1cII had a repeating unit consisting of (1 -> 4)-alpha-D-Glcp and (1 -> 6)-alpha-D-Glcp in a molar ratio of 4:1. Such glucans isolated from A. sinensis (Oliv.) Diets have not been previously reported. (c) 2006 Published by Elsevier Ltd.

引用

页码：1870 / 1877

页数：8

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