Cloning of genomic DNA of rice 5-enolpyruvylshikimate 3-phosphate synthase gene and chromosomal localization of the gene

被引：15

作者：

Xu, JW ^{[1
]}

Feng, DJ ^{[1
]}

Li, XG ^{[1
]}

Chang, TJ ^{[1
]}

Zhu, Z ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

来源：

SCIENCE IN CHINA SERIES C-LIFE SCIENCES | 2002年 / 45卷 / 03期

基金：

中国国家自然科学基金;

关键词：

5-enolpyruvylshikimate 3-phosphate synthase; gene isolation; DNA sequence; chromosomal locating;

D O I：

10.1360/02yc9028

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The shikimate pathway enzyme 5-enolpyruvylshikimate 3-phosphate synthase (EPSPs) is the target of nonselective herbicide glyphosate. A partial rice epsps cDNA was generated by RT-PCR with primers designed according to EST sequence in GenBank and used as probe for rice genomic library screening. In a screen of approximately 8.0x10(4) clones from the rice genomic library, sixteen positive clones were obtained, which strongly hybridized to the probe. One clone, E11, was selected for further analysis and the full-length 3661 by rice epsps genomic sequence was obtained. Sequence analysis and homologous comparison revealed that epsps gene is composed of 8 exons and 7 introns. Analysis by restriction fragment length polymorphism with the probe of rice epsps cDNA fragment confirmed that rice epsps is located on chromosome 6 with an indica-japonica (ZYQ8-JX17) double-haploid (DH) population. This is the first report on the EPSP synthase from monocotyledons.

引用

页码：251 / 259

页数：9