Cell wall associated protein TasA provides an initial binding component to extracellular polysaccharides in dual-species biofilm

被引:21
作者
Duanis-Assaf, Danielle [1 ,2 ]
Duanis-Assaf, Tal [3 ]
Zeng, Guanghong [4 ]
Meyer, Rikke Louise [4 ]
Reches, Meital [3 ]
Steinberg, Doron [2 ]
Shemesh, Moshe [1 ]
机构
[1] ARO, Volcani Ctr, Dept Food Qual & Safety, Inst Postharvest Technol & Food Sci, Rishon Leziyyon, Israel
[2] Hebrew Univ Jerusalem Hadassah Hosp & Med Sch, Fac Dent Med, Inst Dent Sci, Biofilm Res Lab, Jerusalem, Israel
[3] Hebrew Univ Jerusalem, Inst Chem, Jerusalem, Israel
[4] Aarhus Univ, iNANO, Aarhus C, Denmark
关键词
BACILLUS-SUBTILIS; STREPTOCOCCUS-MUTANS; BACTERIA; IDENTIFICATION; MECHANISMS; TARGETS;
D O I
10.1038/s41598-018-27548-1
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Many bacteria in biofilm surround themselves by an extracellular matrix composed mainly of extracellular polysaccharide (EP), proteins such as amyloid-like fibers (ALF) and nucleic acids. While the importance of EP in attachment and acceleration of biofilm by a number of different bacterial species is well established, the contribution of ALF to attachment in multispecies biofilm remains unknown. The study presented here aimed to investigate the role of TasA, a precursor for ALF, in cell-cell interactions in dual-species biofilms of Bacillus subtilis and Streptococcus mutans. Expression of major B. subtilis matrix operons was significantly up-regulated in the presence of S. mutans during different stages of biofilm formation, suggesting that the two species interacted and modulated gene expression in each other. Wild-type B. subtilis expressing TasA adhered strongly to S. mutans biofilm, while a TasA-deficient mutant was less adhesive and consequently less abundant in the dual-species biofilm. Dextran, a biofilm polysaccharide, induced aggregation of B. subtilis and stimulated adhesion to S. mutans biofilms. This effect was only observed in the wild-type strain, suggesting that interactions between TasA and dextran-associated EP plays an important role in inter-species interactions during initial stages of multispecies biofilm development.
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页数:10
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