Interference and co-existence of staphylococci and Cutibacterium acnes within the healthy human skin microbiome

被引:25
作者
Ahle, Charlotte Marie [1 ,2 ]
Stodkilde, Kristian [3 ]
Poehlein, Anja [4 ]
Boemeke, Mechthild [4 ]
Streit, Wolfgang R. [2 ]
Wenck, Horst [1 ]
Reuter, Joern Hendrik [1 ]
Huepeden, Jennifer [1 ]
Brueggemann, Holger [3 ]
机构
[1] Beiersdorf AG, Res & Dev, Front End Innovat, D-20245 Hamburg, Germany
[2] Univ Hamburg, Dept Microbiol & Biotechnol, D-22609 Hamburg, Germany
[3] Aarhus Univ, Dept Biomed, DK-8000 Aarhus, Denmark
[4] Univ Gottingen, Inst Microbiol & Genet, Dept Genom & Appl Microbiol, D-37073 Gottingen, Germany
关键词
PROPIONIBACTERIUM-ACNES; EPIDERMIDIS; AUREUS; IDENTIFICATION; GALLIDERMIN; SHAPE;
D O I
10.1038/s42003-022-03897-6
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Human skin is populated by trillions of microbes collectively called the skin microbiome. Staphylococcus epidermidis and Cutibacterium acnes are among the most abundant members of this ecosystem, with described roles in skin health and disease. However, knowledge regarding the health beneficial effects of these ubiquitous skin residents is still limited. Here, we profiled the staphylococcal and C. acnes landscape across four different skin sites of 30 individuals (120 skin samples) using amplicon-based next-generation sequencing. Relative abundance profiles obtained indicated the existence of phylotype-specific co-existence and exclusion scenarios. Co-culture experiments with 557 staphylococcal strains identified 30 strains exhibiting anti-C. acnes activities. Notably, staphylococcal strains were found to selectively exclude acne-associated C. acnes and co-exist with healthy skin-associated phylotypes, through regulation of the antimicrobial activity. Overall, these findings highlight the importance of skin-resident staphylococci and suggest that selective microbial interference is a contributor to healthy skin homeostasis.
引用
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页数:14
相关论文
共 72 条
[1]   Staphylococcus saccharolyticus: An Overlooked Human Skin Colonizer [J].
Ahle, Charlotte M. ;
Stdkilde, Kristian ;
Afshar, Mastaneh ;
Poehlein, Anja ;
Ogilvie, Lesley A. ;
Soderquist, Bo ;
Huepeden, Jennifer ;
Bruggemann, Holger .
MICROORGANISMS, 2020, 8 (08) :1-12
[2]   Comparison of three amplicon sequencing approaches to determine staphylococcal populations on human skin [J].
Ahle, Charlotte Marie ;
Stodkilde-Jorgensen, Kristian ;
Poehlein, Anja ;
Streit, Wolfgang R. ;
Hupeden, Jennifer ;
Bruggemann, Holger .
BMC MICROBIOLOGY, 2021, 21 (01)
[3]  
[Anonymous], 2009, R Package Version
[4]   Porphyrin Production and Regulation in Cutaneous Propionibacteria [J].
Barnard, Emma ;
Johnson, Tremylla ;
Tracy Ngo ;
Arora, Uma ;
Leuterio, Gunilla ;
McDowell, Andrew ;
Li, Huiying .
MSPHERE, 2020, 5 (01)
[5]   Trimmomatic: a flexible trimmer for Illumina sequence data [J].
Bolger, Anthony M. ;
Lohse, Marc ;
Usadel, Bjoern .
BIOINFORMATICS, 2014, 30 (15) :2114-2120
[6]   Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2 [J].
Bolyen, Evan ;
Rideout, Jai Ram ;
Dillon, Matthew R. ;
Bokulich, NicholasA. ;
Abnet, Christian C. ;
Al-Ghalith, Gabriel A. ;
Alexander, Harriet ;
Alm, Eric J. ;
Arumugam, Manimozhiyan ;
Asnicar, Francesco ;
Bai, Yang ;
Bisanz, Jordan E. ;
Bittinger, Kyle ;
Brejnrod, Asker ;
Brislawn, Colin J. ;
Brown, C. Titus ;
Callahan, Benjamin J. ;
Caraballo-Rodriguez, Andres Mauricio ;
Chase, John ;
Cope, Emily K. ;
Da Silva, Ricardo ;
Diener, Christian ;
Dorrestein, Pieter C. ;
Douglas, Gavin M. ;
Durall, Daniel M. ;
Duvallet, Claire ;
Edwardson, Christian F. ;
Ernst, Madeleine ;
Estaki, Mehrbod ;
Fouquier, Jennifer ;
Gauglitz, Julia M. ;
Gibbons, Sean M. ;
Gibson, Deanna L. ;
Gonzalez, Antonio ;
Gorlick, Kestrel ;
Guo, Jiarong ;
Hillmann, Benjamin ;
Holmes, Susan ;
Holste, Hannes ;
Huttenhower, Curtis ;
Huttley, Gavin A. ;
Janssen, Stefan ;
Jarmusch, Alan K. ;
Jiang, Lingjing ;
Kaehler, Benjamin D. ;
Bin Kang, Kyo ;
Keefe, Christopher R. ;
Keim, Paul ;
Kelley, Scott T. ;
Knights, Dan .
NATURE BIOTECHNOLOGY, 2019, 37 (08) :852-857
[7]   Staphylococcus epidermidis-Skin friend or foe? [J].
Brown, Morgan M. ;
Horswill, Alexander R. .
PLOS PATHOGENS, 2020, 16 (11)
[8]   A Janus-Faced Bacterium: Host-Beneficial and -Detrimental Roles of Cutibacterium acnes [J].
Bruggemann, Holger ;
Salar-Vidal, Llanos ;
Gollnick, Harald P. M. ;
Lood, Rolf .
FRONTIERS IN MICROBIOLOGY, 2021, 12
[9]   The human skin microbiome [J].
Byrd, Allyson L. ;
Belkaid, Yasmine ;
Segre, Julia A. .
NATURE REVIEWS MICROBIOLOGY, 2018, 16 (03) :143-155
[10]  
Callahan BJ, 2016, NAT METHODS, V13, P581, DOI [10.1038/NMETH.3869, 10.1038/nmeth.3869]