Endoplasmic reticulum stress in the livers of BDNF heterozygous knockout mice

被引：7
作者：
Cirrik, Selma [1 ]
Hacioglu, Gulay [2 ]
Abidin, Ismail [3 ]
Aydin-Abidin, Selcen [3 ]
Noyan, Tevfik [4 ]
机构：
[1] Ordu Univ, Fac Med, Dept Physiol, Ordu, Turkey
[2] Giresun Univ, Fac Med, Dept Physiol, Giresun, Turkey
[3] Karadeniz Tech Univ, Fac Med, Dept Biophys, Trabzon, Turkey
[4] Ordu Univ, Fac Med, Dept Med Biochem, Ordu, Turkey
来源：
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY | 2019年 / 125卷 / 04期
关键词：
BDNF heterozygous knockout mice; endoplasmic reticulum stress; apoptosis; steatosis; SREBP-1c; UNFOLDED PROTEIN RESPONSE; NEUROTROPHIC FACTOR BDNF; HEPATIC STEATOSIS; ER STRESS; INSULIN-RESISTANCE; LIPID-METABOLISM; EXPRESSION; ALPHA; DEATH; CHOP;
D O I：
10.1080/13813455.2018.1489850
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Context: Involvement of endoplasmic reticulum (ER) stress and brain-derived neurotrophic factor (BDNF) in hepatic lipid metabolism has been reported previously. Objective: The effects of chronic BDNF deficiency on ER stress response in the livers were examined in this study. Methods: BDNF(+/-) mice, characterised by BDNF deficiency, and their wild-type (WT) littermates were used. The ER stress was induced by tunicamycin (Tm) (0.5 mg/kg, intraperitoneal). Animals were divided into four groups; WT, WT + Tm, BDNF(+/-), and BDNF(+/-)+Tm. Results: At the basal conditions, BDNF deficiency did not affect hepatic cell death or lipid accumulation. However, during ER stress, BDNF(+/-)+Tm group showed increased apoptosis, GADD153 immunostaining, sterol regulatory element-binding protein-1c (SREBP-1c) level, and steatosis compared to the WT + Tm group. Conclusion: Endogenous BDNF might be protective against apoptosis through GADD153 suppression and steatosis via SREBP-1c suppression during ER stress. This effect of BDNF might be clinically important for type 2 diabetes and obesity, which are related with both ER stress and BDNF deficiency.
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收藏
页码：378 / 386
页数：9
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