Murine tissue inhibitor of metalloproteinases-4 (Timp-4): CDNA isolation and expression in adult mouse tissues

被引：170

作者：

Leco, KJ

Apte, SS

Taniguchi, GT

Hawkes, SP

Khokha, R

Schultz, GA

Edwards, DR

机构：

[1] UNIV CALGARY,DEPT MED BIOCHEM,CALGARY,AB T2N 4N1,CANADA

[2] CLEVELAND CLIN FDN,DEPT BIOCHEM ENGN,CLEVELAND,OH 44195

[3] UNIV CALIF SAN FRANCISCO,DEPT BIOPHARMACEUT SCI,SAN FRANCISCO,CA 94143

[4] UNIV CALIF SAN FRANCISCO,DEPT PHARMACEUT CHEM,SAN FRANCISCO,CA 94143

[5] PRINCESS MARGARET HOSP,ONTARIO CANC INST,TORONTO,ON M5G 2M5,CANADA

来源：

FEBS LETTERS | 1997年 / 401卷 / 2-3期

关键词：

tissue inhibitor of metalloproteinases; extracellular matrix; cDNA cloning; gene expression;

D O I：

10.1016/S0014-5793(96)01474-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated cDNA clones corresponding to a new member of the murine tissue inhibitor of metalloproteinase (TIMP) family, designated Timp-4. The nucleotide sequence predicts a protein of 22 609 Da that contains the characteristic 12 cysteine TIMP signature, TIMP-4 is more closely related to TIMP-2 and TIMP-3 than to TIMP-1 (48%, 45% and 38% identity, respectively), Analysis of Timp-4 mRNA expression in adult mouse tissues indicated a 1.2 kb transcript in brain, heart, ovary and skeletal muscle, This pattern of expression distinguishes Timp-4 from other Timps, suggesting that the TIMP-4 protein may be an important tissue-specific regulator of extracellular matrix remodelling.

引用

页码：213 / 217

页数：5

共 31 条

[1]

Alexander CM, 1996, DEVELOPMENT, V122, P1723

[2] GENE ENCODING A NOVEL MURINE TISSUE INHIBITOR OF METALLOPROTEINASES (TIMP), TIMP-3, IS EXPRESSED IN DEVELOPING MOUSE EPITHELIA, CARTILAGE, AND MUSCLE, AND IS LOCATED ON MOUSE CHROMOSOME-10 [J].