Label-free optical-resolution photoacoustic microscopy of superficial microvasculature using a compact visible laser diode excitation

被引：48

作者：

Zeng, Lvming ^{[1
,2
]}

Piao, Zhonglie ^{[2
]}

Huang, Shenghai ^{[2
]}

Jia, Wangcun ^{[2
]}

Chen, Zhongping ^{[2
,3
]}

机构：

[1] Jiangxi Sci & Technol Normal Univ, Key Lab Opt Elect & Commun, Nanchang 330038, Peoples R China

[2] Univ Calif Irvine, Beckman Laser Inst, Irvine, CA 92612 USA

[3] Univ Calif Irvine, Dept Biomed Engn, Irvine, CA 92697 USA

来源：

OPTICS EXPRESS | 2015年 / 23卷 / 24期

基金：

美国国家卫生研究院;

关键词：

IN-VIVO; HIGH-SPEED; CODED EXCITATION; LOW-COST; TOMOGRAPHY; SYSTEM;

D O I：

10.1364/OE.23.031026

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have developed laser-diode-based optical-resolution photoacoustic microscopy (LD-OR-PAM) of superficial microvasculature which has the desirable properties of being compact, low-cost, and label-free. A 300-mW visible pulsed laser diode was operated at a 405 +/- 5 nm wavelength with a pulse energy as low as 52 nJ. By using a 3.6 MHz ultrasound transducer, the system was tested on carbon fibers with a lateral resolution of 0.95 mu m and an SNR of 38 dB. The subcutaneous microvasculature on a mouse back was imaged without an exogenous contrast agent which demonstrates the potential of the proposed prototype for skin chromophores. Our eventual goal is to offer a practical and affordable multi-wavelength functional LD-OR-PAM instrument suitable for clinical applications. (c) 2015 Optical Society of America

引用

页码：31026 / 31033

页数：8

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