Role of the K-Antigen subgroup of capsular polysaccharides in the early recognition process between Rhizobium meliloti and alfalfa leaves

被引:23
作者
BecquartdeKozak, I
Reuhs, BL
Buffard, D
Breda, C
Kim, JS
Esnault, R
Kondorosi, A
机构
[1] CNRS, INST SCI VEGETALES, F-91198 GIF SUR YVETTE, FRANCE
[2] UNIV GEORGIA, COMPLEX CARBOHYDRATE RES CTR, ATHENS, GA 30602 USA
[3] UNIV PARIS 07, F-75005 PARIS, FRANCE
关键词
bacterial surface polysaccharides; gene expression; Medicago sativa;
D O I
10.1094/MPMI.1997.10.1.114
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We used a model system to investigate the induction of plant genes by bacterial surface compounds, We have infiltrated alfalfa leaves with wild-type Rhizobium meliloti strain Rm41 and mutant derivatives, which are deficient in the production of exopolysaccharides (EPS), capsular K-polysaccharides (KPS or K-Antigens), or both. We have shown that wild-type R. meliloti was able to induce transcript accumulation of genes encoding enzymes of the iso-flavonoid biosynthetic pathway: chalcone synthase (CHS), chalcone reductase (CHR), and isoflavone reductase (IFR), Kinetics of these transcript accumulations were biphasic, occurring 0.75 to 1.5 h and 6 to 30 h after treatment. The exoB derivative of Bm41 (strain Ag631), which is unable to produce either EPS I or EPS II, was still able to induce very rapid (45-min posttreatment) transcript accumulation of CNS and CHR genes. These results suggested that EPS were not involved in this induction. We then tested three R. meliloti fix-23 mutants (strains PP699, PP711, and PP671), which were deficient in KPS production as shown by PAGE and immunoblot analyses, In control to the results obtained with the mutant strains, infiltration of alfalfa leaves with purified KPS, isolated from strain AK631, led to a very rapid induction of the CHS and CHR genes. Therefore, we hypothesize that KPS may play a role in the early recognition of rhizobia by the leaf cells.
引用
收藏
页码:114 / 123
页数:10
相关论文
共 48 条
[1]  
BLONDON F, 1961, REV GEN BOT, V71, P293
[2]   BACTERIAL POLYSACCHARIDE CAPSULE SYNTHESIS, EXPORT AND EVOLUTION OF STRUCTURAL DIVERSITY [J].
BOULNOIS, GJ ;
JANN, K .
MOLECULAR MICROBIOLOGY, 1989, 3 (12) :1819-1823
[3]   EXPRESSION OF RHIZOBIUM-LEGUMINOSARUM CFN42 GENES FOR LIPOPOLYSACCHARIDE IN STRAINS DERIVED FROM DIFFERENT R-LEGUMINOSARUM SOIL ISOLATES [J].
BRINK, BA ;
MILLER, J ;
CARLSON, RW ;
NOEL, KD .
JOURNAL OF BACTERIOLOGY, 1990, 172 (02) :548-555
[5]   LIPOPOLYSACCHARIDE MUTANTS OF RHIZOBIUM-MELILOTI ARE NOT DEFECTIVE IN SYMBIOSIS [J].
CLOVER, RH ;
KIEBER, J ;
SIGNER, ER .
JOURNAL OF BACTERIOLOGY, 1989, 171 (07) :3961-3967
[6]  
CREMERS HCJC, 1990, J BIOL CHEM, V265, P21122
[7]   RHIZOBIUM LIPOPOLYSACCHARIDE MODULATES INFECTION THREAD DEVELOPMENT IN WHITE CLOVER ROOT HAIRS [J].
DAZZO, FB ;
TRUCHET, GL ;
HOLLINGSWORTH, RI ;
HRABAK, EM ;
PANKRATZ, HS ;
PHILIPHOLLINGSWORTH, S ;
SALZWEDEL, JL ;
CHAPMAN, K ;
APPENZELLER, L ;
SQUARTINI, A ;
GERHOLD, D ;
ORGAMBIDE, G .
JOURNAL OF BACTERIOLOGY, 1991, 173 (17) :5371-5384
[8]   ISOLATION AND CHARACTERIZATION OF MUTANTS OF RHIZOBIUM-LEGUMINOSARUM BV VICIAE 248 WITH ALTERED LIPOPOLYSACCHARIDES - POSSIBLE ROLE OF SURFACE-CHARGE OR HYDROPHOBICITY IN BACTERIAL RELEASE FROM THE INFECTION THREAD [J].
DEMAAGD, RA ;
RAO, AS ;
MULDERS, IHM ;
GOOSENDEROO, L ;
VANLOOSDRECHT, MCM ;
WIJFFELMAN, CA ;
LUGTENBERG, BJJ .
JOURNAL OF BACTERIOLOGY, 1989, 171 (02) :1143-1150
[9]   INVOLVEMENT OF BACTERIAL POLYSACCHARIDES IN PLANT PATHOGENESIS [J].
DENNY, TP .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1995, 33 :173-197
[10]   RHIZOBIUM-LEGUMINOSARUM EXOPOLYSACCHARIDE MUTANTS - BIOCHEMICAL AND GENETIC ANALYSES AND SYMBIOTIC BEHAVIOR ON 3 HOSTS [J].
DIEBOLD, R ;
NOEL, KD .
JOURNAL OF BACTERIOLOGY, 1989, 171 (09) :4821-4830