Date fruits inhibit hepatocyte apoptosis and modulate the expression of hepatocyte growth factor, cytochrome P450 2E1 and heme oxygenase-1 in carbon tetrachloride-induced liver fibrosis

被引:6
作者
Al-Rasheed, Nouf M. [1 ]
Attia, Hala A. [1 ,2 ]
Mohamad, Raeesa A. [3 ,4 ]
Al-Rasheed, Nawal M. [1 ]
Al Fayez, Musaed [3 ]
Al-Amin, Maha A. [1 ]
机构
[1] King Saud Univ, Coll Pharm, Dept Pharmacol & Toxicol, POB 2454, Riyadh 11451, Saudi Arabia
[2] Mansoura Univ, Coll Pharm, Dept Biochem, Mansoura, Egypt
[3] King Saud Univ, Fac Med, Dept Anat, Riyadh, Saudi Arabia
[4] Cairo Univ, Fac Med, Dept Histol, Cairo, Egypt
关键词
Liver fibrosis; apoptosis; hepatocyte growth factor; cytochrome P450 2E1; casapase-3; PHOENIX-DACTYLIFERA L; CHEMICAL-COMPOSITION; TGF-BETA; INDUCTION; PROTECTS; EXTRACT; ANTIOXIDANT; HEPATOTOXICITY; CIRRHOSIS; INJURY;
D O I
10.1080/13813455.2016.1251945
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Context: Date fruits have protective effects against liver fibrosis; however their anti-apoptotic effects have not been investigated. Objective: To investigate the modulating effects of date fruits on pro-and anti-apoptotic markers, cytochrome P450 2E1 (CYP2E1) and hepatocyte growth factor (HGF) in liver fibrosis. Materials and methods: Liver fibrosis was induced by injection of carbon tetrachloride (CCl4) for eight weeks. Date flesh extract (DFE) and pits extract (DPE) were taken daily concomitant with CCl4. Hepatocyte apoptosis was determined by measuring the expression of Fas, caspase-3, Bax, Bcl2 and hemeoxygenase-1 (HO-1). Hepatic levels of HGF and CYP2E1 were determined. Results: Treatment with DFE and DPE significantly attenuated the elevated levels of Fas, caspase 3, Bax and CYP2E1 induced by CCl4. In addition, they alleviated the reduction in Bcl2, HGF and HO-1, the cytoprotective and anti-apoptotic factors in liver. Conclusions DFE and DPE treatment can ameliorate liver fibrosis by inhibiting hepatocyte apoptosis.
引用
收藏
页码:78 / 92
页数:15
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