Immunohistochemical and RT-PCR detection of Na+-dependent inorganic phosphate cotransporter (NaPi-2) in rat brain

被引：28
作者：
Hisano, S [1 ]
Haga, H [1 ]
Li, ZN [1 ]
Tatsumi, S [1 ]
Miyamoto, K [1 ]
Takeda, E [1 ]
Fukui, Y [1 ]
机构：
[1] UNIV TOKUSHIMA,SCH MED,DEPT CLIN NUTR,TOKUSHIMA 770,JAPAN
来源：
BRAIN RESEARCH | 1997年 / 772卷 / 1-2期
关键词：
inorganic phosphate transporter; NaPi-2; reverse transcription-polymerase chain reaction (RT-PCR); immunohistochemistry; nonpyramidal neuron; rat brain;
D O I：
10.1016/S0006-8993(97)00884-6
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Expression of a renal Na+-dependent inorganic phosphate (P-i) cotransporter (NaPi-2) was studied in rat forebrain with reverse transcription and polymerase chain reaction (RT-PCR) and immunohistochemistry. RT-PCR analysis for total RNA from whole brain and sequencing of the PCR products showed expression of NaPi-2 mRNA in the brain. Immunohistochemical analysis revealed NaPi-2 staining in many nonpyramidal neurons of all six layers throughout neocortical areas and in neurons of proisocortical and periallocortical areas. NaPi-2-immunoreactive neurons were also detectable in the piriform cortex, hippocampal formation, caudate-putamen, amygdaloid nuclei and lateral geniculate nucleus. Furthermore, NaPi-2 staining was shown in ependymal cells and microvascular endothelial cells. The present results suggest that NaPi-2 is synthesized within the brain and involved in maintaining P-i homeostasis of certain neurons and/or the entire brain. (C) 1997 Elsevier Science B.V.
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页码：149 / 155
页数：7
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